Evidence map›Paper›PMID 28934663›Full record

ArticleColloids and surfaces. B, Biointerfaces2017

Using a fluorescence quenching method to detect DNA adsorption onto single-walled carbon nanotube surfaces.

Kazuo Umemura, Shizuma Sato, Gilbert Bustamante, Jing Yong Ye

Open access · greenAbstract read
In one paragraph

Article in Colloids and surfaces. B, Biointerfaces, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Redox dye-mediated fluorescence energy transfer of carbon nanotube-based nanosensors.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Kazuo UmemuraBiophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan. Electronic address: meicun2006@163.com.
Shizuma SatoBiophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
Gilbert BustamanteDepartment of Biomedical Engineering, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
Jing Yong YeDepartment of Biomedical Engineering, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
The University of Texas at San Antonio · USTokyo University of Science · JP

Funding

UTSA RISE Research Training ProgramR25GM060655 · NIGMS · UNIVERSITY OF TEXAS SAN ANTONIO · PI BAREA-RODRIGUEZ, EDWIN J · 2000 to 2022
$21.6M
Novel Optoacoustic Sensors with an Open Cavity ConfigurationR21EB008765 · NIBIB · UNIVERSITY OF TEXAS SAN ANTONIO · PI YE, JING YONG · 2008 to 2009
$394k
NIBIB NIH HHS R21 EB008765NIGMS NIH HHS R25 GM060655
6 · The paper itself

Abstract

Surface modification of single-walled carbon nanotubes (SWNTs) with DNA molecules has attracted much attention in recent years to increase SWNT solubility and make various SWNT-based nanobiodevices. Therefore, there is a critical need to quantify the interaction between DNA molecules and SWNT surfaces, particularly the intermediate structures during DNA adsorption. In this study, we demonstrate the ability to detect the adsorption of DNA oligomers on SWNT surfaces by fluorescence spectroscopy. Fluorescein-labelled, 30mer, thymine oligonucleotides (F-T30) were employed as a fluorescent probe to study the interaction of DNA with SWNTs. A clear quenching effect was observed when F-T30 was adsorbed onto SWNT surfaces. Using this method, the amount of DNA adsorbed onto the SWNT surfaces was measured under different sonication conditions to correlate adsorption efficiency with sonication strength and duration. When a bath-type sonicator was used, mild adsorption of F-T30 on SWNT surfaces was observed. Furthermore, a two-step adsorption was observed in this condition. In contrast, we observed rapid adsorption of F-T30 to SWNT surfaces at the higher sonication amplitude (60% maximal) using a probe-type sonicator, while only slight adsorption of DNA molecules was observed at the lower amplitude (20% maximal). Our data revealed that the quenching effect can be used to evaluate DNA adsorption onto SWNT surfaces. In addition, atomic force microscopy (AFM) and photoacoustic spectroscopy (PAS) were conducted to provide complementary information on the DNA-SWNT nanoconjugates.

Indexed as

AdsorptionDNAFluoresceinFluorescent DyesKineticsNanoconjugatesNanotubes, CarbonOligonucleotidesSonicationStaining and LabelingDNAFluoresceinFluorescent DyesNanoconjugatesNanotubes, CarbonOligonucleotidesAtomic force microscopyDnaFluorescence quenchingfluorescence spectroscopyPhotoacoustic spectroscopySingle-walled carbon nanotube

Identifiers

PMID28934663
PMCPMC5714673
OpenAlexW2756123225

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.