Evidence map›Paper›PMID 28934503›Full record

ArticleNucleic acids research2017

A novel requirement for DROSHA in maintenance of mammalian CG methylation.

Athanasia Stathopoulou, Jyoti B Chhetri, John C Ambrose, Pierre-Olivier Estève, Lexiang Ji, Hediye Erdjument-Bromage, Guoqiang Zhang, Thomas A Neubert, Sriharsa Pradhan, Javier Herrero and 2 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
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  7. Epigenetic dynamics in cancer stem cell dormancy.Cancer metastasis reviews · 2020
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Athanasia StathopoulouDepartment of Cancer Biology, UCL Cancer Institute, London WC1E 6BT, UK.
Jyoti B ChhetriDepartment of Cancer Biology, UCL Cancer Institute, London WC1E 6BT, UK.
John C AmbroseBill Lyons Informatics Centre, UCL Cancer Institute, London WC1E 6BT, UK.
Pierre-Olivier EstèveNew England Biolabs, 240 Country Road, Ipswich, MA 01938, USA.
Lexiang JiInstitute of Bioinformatics, University of Georgia, 120 East Green Street, Athens, GA 30602, USA.
Hediye Erdjument-BromageDepartment of Biochemistry and Molecular Pharmacology, Skirball Institute, NYU School of Medicine, New York, NY 10016, USA.
Guoqiang ZhangNew England Biolabs, 240 Country Road, Ipswich, MA 01938, USA.
Thomas A NeubertDepartment of Biochemistry and Molecular Pharmacology, Skirball Institute, NYU School of Medicine, New York, NY 10016, USA.
Sriharsa PradhanNew England Biolabs, 240 Country Road, Ipswich, MA 01938, USA.
Javier HerreroBill Lyons Informatics Centre, UCL Cancer Institute, London WC1E 6BT, UK.
Robert J SchmitzInstitute of Bioinformatics, University of Georgia, 120 East Green Street, Athens, GA 30602, USA.
Steen K T OoiDepartment of Cancer Biology, UCL Cancer Institute, London WC1E 6BT, UK.
CRUK Lung Cancer Centre of Excellence · GBNew England Biolabs (United States) · USNew York University · USUniversity College London · GBUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In mammals, faithful inheritance of genomic methylation patterns ensures proper gene regulation and cell behaviour, impacting normal development and fertility. Following establishment, genomic methylation patterns are transmitted through S-phase by the maintenance methyltransferase Dnmt1. Using a protein interaction screen, we identify Microprocessor component DROSHA as a novel DNMT1-interactor. Drosha-deficient embryonic stem (ES) cells display genomic hypomethylation that is not accounted for by changes in the levels of DNMT proteins. DNMT1-mediated methyltransferase activity is also reduced in these cells. We identify two transcripts that are specifically upregulated in Drosha- but not Dicer-deficient ES cells. Regions within these transcripts predicted to form stem-loop structures are processed by Microprocessor and can inhibit DNMT1-mediated methylation in vitro. Our results highlight DROSHA as a novel regulator of mammalian DNA methylation and we propose that DROSHA-mediated processing of RNA is necessary to ensure full DNMT1 activity. This adds to the DROSHA repertoire of non-miRNA dependent functions as well as implicating RNA in regulating DNMT1 activity and correct levels of genomic methylation.

Indexed as

DNA MethylationAnimalsCells, CulturedCRISPR-Cas SystemsCytosineDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesEmbryonic Stem CellsHEK293 CellsHumansMiceRibonuclease IIIRNA, MessengerUp-RegulationCytosineDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNMT1 protein, humanDnmt1 protein, mouseDrosha protein, mouseRibonuclease IIIRNA, Messenger

Identifiers

PMID28934503
PMCPMC5766157
OpenAlexW4318470750

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.