ArticlePloS one2017
Light entrainment of the murine intraocular pressure circadian rhythm utilizes non-local mechanisms.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Temporal Variations in Inflammatory-Related Ocular Surface Parameters: A Systematic Review and Meta-Analysis.Investigative ophthalmology & visual science · 2026Pooled it
- Adrenergic Pathways Mediate Circadian IOP Rhythm in Rats.Investigative ophthalmology & visual science · 2026Article
- Additive framework of hormonal waves accounts for species and age differences in circadian intraocular pressure rhythm.Npj biological timing and sleep · 2026Article
- Opposing Roles of OPN3 and OPN5 Affecting the Intrinsic Pupillary Light Reflex of the Mammalian Iris.Investigative ophthalmology & visual science · 2026Article
- Circadian IOP Rhythm in Rats Is Driven by Neural Signals From the Brain.Investigative ophthalmology & visual science · 2026Article
- IMI: The Role of Light in Refractive Development and Myopia: Evidence from Animal and Human Studies.Investigative ophthalmology & visual science · 2025Review
- Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse.Eye and vision (London, England) · 2025Article
- TRPV4 controls circadian and pathological ocular hypertension.The Journal of physiology · 2025Article
- Circadian Rhythms in Murine Ocular Tissues Including Sclera Are Affected by Neurobasal A Medium Preincubation and Mouse Strain, but Not Sex.Investigative ophthalmology & visual science · 2025Article
- Neuropsin, TRPV4 and intracellular calcium mediate intrinsic photosensitivity in corneal epithelial cells.The ocular surface · 2025Article
- Disruption of circadian intraocular pressure fluctuations in mice by the Lyst beige-J mutation.Experimental eye research · 2025Article
- Melanopsin in the human and chicken choroid.Experimental eye research · 2024Article
- Effect of Ambient Lighting on Intraocular Pressure Rhythms in Rats.Investigative ophthalmology & visual science · 2024Article
- TRPV4 subserves physiological and pathological elevations in intraocular pressure.Research square · 2024Article
- Sleep deprivation induces corneal endothelial dysfunction by downregulating Bmal1.BMC ophthalmology · 2024Article
- Circadian rhythm of intraocular pressure.The journal of physiological sciences : JPS · 2024Review
- Encephalopsin (OPN3) is required for normal refractive development and the GO/GROW response to induced myopia.Molecular vision · 2023Article
- A portable feedback-controlled pump for monitoring eye outflow facility in conscious rats.PloS one · 2023Article
- Suppression of trabecular meshwork phagocytosis by norepinephrine is associated with nocturnal increase in intraocular pressure in mice.Communications biology · 2022Article
- Circadian Regulation of Retinal Pigment Epithelium Function.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeIntraocular pressure (IOP) is known to have a strong circadian rhythm, yet how light/dark cycles entrain this rhythm is unknown. The purpose of this study was to assess whether, like the retina, the mammalian ciliary body and IOP clocks have an intrinsic ability to entrain to light/dark cycles.
methodsIris-ciliary body complexes were obtained from period2:luciferase (PER2::LUC) mice and cultured to measure bioluminescence rhythmicity. Pairs of the iris-ciliary body complex were exposed to antiphasic 9:15 h light/dark cycle in vitro. After 4 days of exposure to light/dark cycles, bioluminescence was recorded to establish their circadian phases. In addition, pairs of the iris-ciliary body complex co-cultured with the retinas or corneas of wild-type mice were also investigated. The IOP circadian changes of free-running Opn4-/-;rd1/rd1 mice whose behavior was antiphasic to wild-type were measured by a rebound tonometry, and compared with wild-type mice. Opn3, Opn4, and Opn5 mRNA expression in the iris-ciliary body were analyzed using RT-PCR.
resultsThe iris/ciliary body complex expressed Opn3, Opn4, and Opn5 mRNA; however, unlike in retina and cornea, neither the iris-CB complex nor the co-cultured complex was directly entrained by light-dark cycle in vitro. The diurnal IOP change of Opn4-/-;rd1/rd1 mice showed an antiphasic pattern to wild-type mice and their rhythms followed the whole-animal behavioral rhythm.
conclusionsDespite expressing mRNA for several non-visual opsins, circadian rhythms of the iris-ciliary body complex of mice do not entrain directly to light-dark cycles ex vivo. Unlike retina, the iris/ciliary body clocks of blind mice remain synchronized to the organismal behavioral rhythm rather than local light-dark cycles. These results suggest that IOP rhythm entrainment is mediated by a systemic rather than local signal in mice.
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