ArticlePloS one2017
Angiopoietin-like protein 2 regulates Porphyromonas gingivalis lipopolysaccharide-induced inflammatory response in human gingival epithelial cells.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Increased Production of Angiopoietin-like Protein 2 in a Ligature- and LPS-Induced Periodontitis Mouse Model May Promote Colorectal Tumor Progression.Journal of clinical medicine · 2026Article
- Removal of CD5 on T cells alters their differentiation and cytokine production in anFrontiers in immunology · 2026Article
- Bactericidal and Anti-Inflammatory Effects ofVeterinary sciences · 2025Article
- Estrogen prevented gingival barrier injury fromInfection and immunity · 2025Article
- Roles of intralesional bacteria in the initiation and progression of oral squamous cell carcinoma.Cancer medicine · 2024Review
- Periodontitis promotes hepatocellular carcinoma in Stelic Animal model (STAM) mice.Scientific reports · 2024Article
- Interleukin-1β regulates odontogenic ameloblast-associated protein gene transcription in human gingival epithelial cells.Odontology · 2022Article
- Role of Porphyromonas gingivalis in oral and orodigestive squamous cell carcinoma.Periodontology 2000 · 2022Review
- Circulating Exosomal miR-221 from Maternal Obesity Inhibits Angiogenesis via TargetingInternational journal of molecular sciences · 2021Article
- Drift of the Subgingival Periodontal Microbiome during Chronic Periodontitis in Type 2 Diabetes Mellitus Patients.Pathogens (Basel, Switzerland) · 2021Article
- Integrin α5β1 inhibition by ATN-161 reduces neuroinflammation and is neuroprotective in ischemic stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Article
- S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells.BioMed research international · 2020Article
- Effect of ANGPTL7 on Proliferation and Differentiation of MC3T3-E1 Cells.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- 1,25-dihydroxyvitamin DBMC oral health · 2019Article
- Vitamin D/VDR signaling inhibits LPS-induced IFNγ and IL-1β in Oral epithelia by regulating hypoxia-inducible factor-1α signaling pathway.Cell communication and signaling : CCS · 2019Article
- Downregulation of angiopoietin-like protein 2 inhibits cementoblast differentiation partially by activating the ERK1/2 signaling pathway.American journal of translational research · 2019Article
- Oral Mucosal Epithelial Cells.Frontiers in immunology · 2019Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Angiopoietin-like protein 2 (ANGPTL2) maintains tissue homeostasis by inducing inflammation and angiogenesis. It is produced in infiltrating immune cells or resident cells, such as adipocytes, vascular endothelial cells, and tumor cells. We hypothesized that ANGPTL2 might play an important role as a unique mediator in both systemic and periodontal disease. We demonstrated an increased ANGPTL2 concentration in gingival crevicular fluid from chronic periodontitis patients. Porphyromonas gingivalis lipopolysaccharide (LPS) treatment strongly induced ANGPTL2 mRNA and protein levels in Ca9-22 human gingival epithelial cells. Recombinant human ANGPTL2 increased interleukin 1β (IL-1β), IL-8, and tumor necrosis factor-α (TNF-α) mRNA and protein levels in Ca9-22 cells. Small-interfering (si)RNA-mediated ANGPTL2 knockdown in Ca9-22 cells reduced IL-1β, IL-8 and TNF-α mRNA and protein levels compared with control siRNA (p<0.01) in P. gingivalis LPS-stimulated Ca9-22 cells. Antibodies against integrin α5β1, an ANGPTL receptor, blocked induction of these inflammatory cytokines in P. gingivalis LPS-treated Ca9-22 cells, suggesting that secreted ANGPTL induces inflammatory cytokines in gingival epithelial cells via an autocrine loop. The classic sequential cascade of P. gingivalis LPS → inflammatory cytokine induction is well established. However, in the current study, we reveal a novel cascade comprising sequential P. gingivalis LPS → ANGPTL2 → integrin α5β1 → inflammatory cytokine induction, which might be responsible for inducing potent periodontal disorganization activity in gingival epithelial cells. Via this pathway, ANGPTL2 functions in the pathogenesis of periodontitis and contributes to prolonging chronic inflammation in patients with systemic disease.
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