ArticleWorld journal of microbiology & biotechnology2017
RNAi-mediated down-regulation of a melanin polyketide synthase (pks1) gene in the fungus Slafractonia leguminicola.
Article in World journal of microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 17 citations in OpenAlex.
- Endophytic Alternaria oxytropis modulates host metabolism and enhances stress resilience in locoweed independent of swainsonine biosynthesis.BMC plant biology · 2026Article
- siRNA-Silencing ofFrontiers in microbiology · 2025Article
- Analysis of the Mycotoxin Levels and Expression Pattern of SWN Genes at Different Time Points in the FungusMicroorganisms · 2024Article
- Expanding the Toolbox for Genetic Manipulation inJournal of fungi (Basel, Switzerland) · 2024Article
- swnk plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae.Biotechnology letters · 2023Article
- Silencing of the Transmembrane Transporter (Journal of fungi (Basel, Switzerland) · 2023Article
- Mycoviral gene integration converts a plant pathogenic fungus into a biocontrol agent.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Phylogenetic Comparison of Swainsonine Biosynthetic Gene Clusters among Fungi.Journal of fungi (Basel, Switzerland) · 2022Article
- Genetic Relationships in the Toxin-Producing Fungal Endophyte,Journal of fungi (Basel, Switzerland) · 2021Article
- Vib-PT, an Aromatic Prenyltransferase Involved in the Biosynthesis of Vibralactone fromApplied and environmental microbiology · 2020Article
- A comprehensive catalogue of polyketide synthase gene clusters in lichenizing fungi.Journal of industrial microbiology & biotechnology · 2018Review
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4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fungus Slafractonia leguminicola, the causal agent of blackpatch disease of legumes produces two mycotoxins slaframine and swainsonine, causing slobbers' symptoms and locoism of grazing animals, respectively. The genetics of this important fungus is poorly understood. This work aimed to develop a genetic transformation system and evaluate the efficacy of RNA interference (RNAi) in S. leguminicola. In this study, S. leguminicola was transformed using a PEG-mediated method with a fungal construct that carries a hygromycin resistance cassette. To assess the use of RNAi, a silencing construct pSilentPKS1-AS was constructed which includes inverted repeat transgenes of the polyketide synthase gene (pks1) that is involved in melanin biosynthesis. Transformation of S. leguminicola with the IRT pks1 vector decreased pks1 transcripts levels 82-92% in knockdown mutants when compared with the wild type and was accompanied with a reduction in melanin and swainsonine production. These results demonstrate that RNAi can be a useful tool for studying gene function in S. leguminicola.
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