Evidence map›Paper›PMID 28932081›Full record

ArticleWorld journal of gastroenterology2017

(-)-Epigallocatechin-3-gallate enhances poly I:C-induced interferon-λ1 production and inhibits hepatitis C virus replication in hepatocytes.

Yi-Zhong Wang, Jie-Liang Li, Xu Wang, Ting Zhang, Wen-Zhe Ho

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Yi-Zhong WangDepartment of Infectious Diseases, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China.
Jie-Liang LiDepartment of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, United States.
Xu WangDepartment of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, United States.
Ting ZhangDepartment of Infectious Diseases, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China. zhangt@shchildren.com.cn.
Wen-Zhe HoDepartment of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, United States.
Temple University · USCreative Research Enterprises (United States) · USShanghai Children's Hospital · CNShanghai Jiao Tong University · CN

Funding

Opioids, HIV/HCV and Host Cell Innate ImmunityR01DA022177 · NIDA · TEMPLE UNIV OF THE COMMONWEALTH · PI HO, WENZHE · 2007 to 2017
$2.9M
NIDA NIH HHS R01 DA022177
6 · The paper itself

Abstract

aimTo investigate the effect of (-)-epigallocatechin-3-gallate (EGCG) on polyinosinic-polycytidylic acid (poly I:C)-triggered intracellular innate immunity against hepatitis C virus (HCV) in hepatocytes.

methodsA cell culture model of HCV infection was generated by infecting a hepatoma cell line, Huh7, with HCV JFH-1 strain (JFH-1-Huh7). Poly I:C with a high molecular weight and EGCG were used to stimulate the JFH-1-Huh7 cells. Real-time reverse transcription-polymerase chain reaction was used to detect the expression levels of intracellular mRNAs and of intracellular and extracellular HCV RNA. Enzyme-linked immunosorbent assay was used to evaluate the interferon (IFN)-λ1 protein level in the cell culture supernatant. Immunostaining was used to examine HCV core protein expression in Huh7 cells.

resultsOur recent study showed that HCV replication could impair poly I:C-triggered intracellular innate immune responses in hepatocytes. In the current study, we showed that EGCG treatment significantly increased the poly I:C-induced expression of Toll-like receptor 3 (TLR3), retinoic acid-inducible gene I, and IFN-λ1 in JFH-1-Huh7 cells. In addition, supplementation with EGCG increased the poly I:C-mediated antiviral activity in JFH-1-Huh7 cells at the intracellular and extracellular HCV RNA and protein levels. Further investigation of the mechanisms showed that EGCG treatment significantly enhanced the poly I:C-induced expression of IFN-regulatory factor 9 and several antiviral IFN-stimulated genes, including

conclusionOur observations provide experimental evidence that EGCG has the ability to enhance poly I:C-induced intracellular antiviral innate immunity against HCV replication in hepatocytes.

Indexed as

Antiviral AgentsCatechinCell Line, TumorDEAD Box Protein 58Enzyme-Linked Immunosorbent AssayHepacivirusHepatitis CHepatocytesHumansImmunity, InnateInterferon-gammaInterferon-Stimulated Gene Factor 3, gamma SubunitPoly I-CReceptors, ImmunologicReverse Transcriptase Polymerase Chain ReactionRNA, ViralAntiviral AgentsCatechinDEAD Box Protein 58epigallocatechin gallateIFNG protein, humanInterferon-gammaInterferon-Stimulated Gene Factor 3, gamma SubunitIRF9 protein, humannucleocapsid protein, Hepatitis C virusPoly I-CReceptors, ImmunologicRIGI protein, humanRNA, ViralTLR3 protein, humanToll-Like Receptor 3Viral Core Proteins(-)-Epigallocatechin-3-gallateHepatitis C virusIFN-stimulated genesIFN-λ1Retinoic acid-inducible gene IToll-like receptor 3

Identifiers

PMID28932081
PMCPMC5583574
OpenAlexW2749675126

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.