Evidence map›Paper›PMID 28931831›Full record

ArticleScientific reports2017

Hyperhomocysteinemia Alters Retinal Endothelial Cells Barrier Function and Angiogenic Potential via Activation of Oxidative Stress.

Riyaz Mohamed, Isha Sharma, Ahmed S Ibrahim, Heba Saleh, Nehal M Elsherbiny, Sadanand Fulzele, Khaled Elmasry, Sylvia B Smith, Mohamed Al-Shabrawey, Amany Tawfik

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 64 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Riyaz MohamedDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Isha SharmaDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Ahmed S IbrahimDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Heba SalehDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Nehal M ElsherbinyDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Sadanand FulzeleDepartment: Orthopedic Surgery, MCG, Augusta University, Augusta, GA, USA.
Khaled ElmasryDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Sylvia B SmithJames and Jean Culver Vision Discovery Institute, Medical College of Georgia (MCG), Augusta University, Augusta, USA.
Mohamed Al-ShabraweyDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Amany TawfikDepartment of Oral Biology and Anatomy, Dental College of Georgia, Augusta University, Augusta, GA, USA. amtawfik@augusta.edu.
Augusta University · USDiscovery Institute · USMansoura University · EG

Funding

Role of homocysteine in retinal diseaseR01EY012830 · NEI · MEDICAL COLLEGE OF GEORGIA (MCG) · PI SMITH, SYLVIA B. · 2000 to 2016
$4.4M
Role of lipoxygenase pathway in early microvascular dysfunction during diabetic rR01EY023315 · NEI · AUGUSTA UNIVERSITY · PI AL-SHABRAWEY, MOHAMED AL-SAYED · 2013 to 2017
$1.8M
NEI NIH HHS R01 EY012830NEI NIH HHS R01 EY023315
6 · The paper itself

Abstract

Hyperhomocysteinemia (HHcy) is associated with several human visual disorders, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD). Breakdown of the blood-retinal barrier (BRB) is linked to vision loss in DR and AMD. Our previous work revealed that HHcy altered BRB in retinal endothelial cells in vivo. Here we hypothesize that homocysteine (Hcy) alters retinal endothelial cell barrier function and angiogenic potential via activation of oxidative stress. Human retinal endothelial cells (HRECs) treated with and without different concentrations of Hcy showed a reduction of tight junction protein expression, increased FITC dextran leakage, decreased transcellular electrical resistance and increased angiogenic potential. In addition, HRECs treated with Hcy showed increased production of reactive oxygen species (ROS). The anti-oxidant N-acetyl-cysteine (NAC) reduced ROS formation and decreased FITC-dextran leakage in Hcy treated HRECs. A mouse model of HHcy, in which cystathionine-β-synthase is deficient (cbs

Indexed as

Oxidative StressAnimalsCells, CulturedDisease Models, AnimalEndothelial CellsHumansHyperhomocysteinemiaMice, Inbred C57BLNeovascularization, PathologicPermeabilityRetinaRetinal Diseases

Identifiers

PMID28931831
PMCPMC5607263
OpenAlexW2754339716

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.