ArticleMolecular biology of the cell2017
Micropipette force probe to quantify single-cell force generation: application to T-cell activation.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Actin conformation dynamics precede force generation in cytotoxic T lymphocytes.Communications biology · 2026Article
- Inference of the 3D pressure field exerted by a single cell from a thin membrane transverse deformation.The European physical journal. E, Soft matter · 2026Article
- Immunological synapse: structures, molecular mechanisms and therapeutic implications in disease.Signal transduction and targeted therapy · 2025Review
- Soft matter mechanics of immune cell aggregates.Journal of the Royal Society, Interface · 2025Article
- Mechanical control of antigen detection and discrimination by T and B cell receptors.Biophysical journal · 2024Review
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- Mechanical forces amplify TCR mechanotransduction in T cell activation and function.Applied physics reviews · 2024Review
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
- Biomembrane force probe (BFP): Design, advancements, and recent applications to live-cell mechanobiology.Exploration (Beijing, China) · 2023Review
- Distinct timing of neutrophil spreading and stiffening during phagocytosis.Biophysical journal · 2022Article
- May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology.Frontiers in immunology · 2022Review
- Mechanosurveillance: Tiptoeing T Cells.Frontiers in immunology · 2022Review
- Mechanotransduction as a major driver of cell behaviour: mechanisms, and relevance to cell organization and future research.Open biology · 2021Review
- Programmable DNA-augmented hydrogels for controlled activation of human lymphocytes.Nanomedicine : nanotechnology, biology, and medicine · 2021Article
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- Non-invasive acquisition of mechanical properties of cells via passive microfluidic mechanisms: A review.Biomicrofluidics · 2021Review
- Controlling T cells spreading, mechanics and activation by micropatterning.Scientific reports · 2021Article
- Determination by Relaxation Tests of the Mechanical Properties of Soft Polyacrylamide Gels Made for Mechanobiology Studies.Polymers · 2021Article
- Controllable Cell Deformation Using Acoustic Streaming for Membrane Permeability Modulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Mouse T cell priming is enhanced by maturation-dependent stiffening of the dendritic cell cortex.eLife · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In response to engagement of surface molecules, cells generate active forces that regulate many cellular processes. Developing tools that permit gathering mechanical and morphological information on these forces is of the utmost importance. Here we describe a new technique, the micropipette force probe, that uses a micropipette as a flexible cantilever that can aspirate at its tip a bead that is coated with molecules of interest and is brought in contact with the cell. This technique simultaneously allows tracking the resulting changes in cell morphology and mechanics as well as measuring the forces generated by the cell. To illustrate the power of this technique, we applied it to the study of human primary T lymphocytes (T-cells). It allowed the fine monitoring of pushing and pulling forces generated by T-cells in response to various activating antibodies and bending stiffness of the micropipette. We further dissected the sequence of mechanical and morphological events occurring during T-cell activation to model force generation and to reveal heterogeneity in the cell population studied. We also report the first measurement of the changes in Young's modulus of T-cells during their activation, showing that T-cells stiffen within the first minutes of the activation process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.