ArticleMolecular biology of the cell2017
Nonrandom γ-TuNA-dependent spatial pattern of microtubule nucleation at the Golgi.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Insights into Microtubule Nucleation at the Golgi Apparatus.Sub-cellular biochemistry · 2026Review
- A structured framework of cytoskeletal proteins and noncentrosomal microtubules promotes the initiation and elongation of invadopodia.Molecular biology of the cell · 2025Article
- CLASP1/2 regulate immune synapse maturation in natural killer cells.Journal of leukocyte biology · 2025Article
- Impact of the Microtubule Cytoskeleton on Insulin Transport in Beta Cells: A 3D Computational Study.bioRxiv : the preprint server for biology · 2025Article
- Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG.Nature communications · 2023Article
- Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines.International journal of molecular sciences · 2022Article
- Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells.Molecular biology of the cell · 2022Article
- Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly.Journal of cell science · 2021Article
- Molecular insight into how γ-TuRC makes microtubules.Journal of cell science · 2021Review
- Microtubules Regulate Localization and Availability of Insulin Granules in Pancreatic Beta Cells.Biophysical journal · 2020Article
- Detection of Microtubule Nucleation Hotspots at the Golgi.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Regulation of Glucose-Dependent Golgi-Derived Microtubules by cAMP/EPAC2 Promotes Secretory Vesicle Biogenesis in Pancreatic β Cells.Current biology : CB · 2019Article
- Misplaced Golgi Elements Produce Randomly Oriented Microtubules and Aberrant Cortical Arrays of Microtubules in Dystrophic Skeletal Muscle Fibers.Frontiers in cell and developmental biology · 2019Article
- A New Look at the Functional Organization of the Golgi Ribbon.Frontiers in cell and developmental biology · 2019Article
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6 authors.
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Abstract
Noncentrosomal microtubule (MT) nucleation at the Golgi generates MT network asymmetry in motile vertebrate cells. Investigating the Golgi-derived MT (GDMT) distribution, we find that MT asymmetry arises from nonrandom nucleation sites at the Golgi (hotspots). Using computational simulations, we propose two plausible mechanistic models of GDMT nucleation leading to this phenotype. In the "cooperativity" model, formation of a single GDMT promotes further nucleation at the same site. In the "heterogeneous Golgi" model, MT nucleation is dramatically up-regulated at discrete and sparse locations within the Golgi. While MT clustering in hotspots is equally well described by both models, simulating MT length distributions within the cooperativity model fits the data better. Investigating the molecular mechanism underlying hotspot formation, we have found that hotspots are significantly smaller than a Golgi subdomain positive for scaffolding protein AKAP450, which is thought to recruit GDMT nucleation factors. We have further probed potential roles of known GDMT-promoting molecules, including γ-TuRC-mediated nucleation activator (γ-TuNA) domain-containing proteins and MT stabilizer CLASPs. While both γ-TuNA inhibition and lack of CLASPs resulted in drastically decreased GDMT nucleation, computational modeling revealed that only γ-TuNA inhibition suppressed hotspot formation. We conclude that hotspots require γ-TuNA activity, which facilitates clustered GDMT nucleation at distinct Golgi sites.
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