ArticleScientific reports2017
The phosphorylation of a kinetochore protein Dam1 by Aurora B/Ipl1 kinase promotes chromosome bipolar attachment in yeast.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Using Yeast Two-Hybrid Screening and Structural Modeling to Identify Candidate Hrr25 Kinase Interactors at the Meiotic Kinetochore inInternational journal of molecular sciences · 2026Article
- Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation.PLoS genetics · 2025Article
- Evidence of 14-3-3 proteins contributing to kinetochore integrity and chromosome congression during mitosis.Journal of cell science · 2024Article
- A communication hub for phosphoregulation of kinetochore-microtubule attachment.Current biology : CB · 2024Article
- Chromosome segregation: Mps1 and Dam1 battle to bind a shared interaction site at the kinetochore.Current biology : CB · 2024Article
- A new layer of regulation of chromosomal passenger complex (CPC) translocation in budding yeast.Molecular biology of the cell · 2023Article
- Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.Open biology · 2023Article
- Article
- Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.The Journal of cell biology · 2022Article
- Yeast Fin1-PP1 dephosphorylates an Ipl1 substrate, Ndc80, to remove Bub1-Bub3 checkpoint proteins from the kinetochore during anaphase.PLoS genetics · 2021Article
- Hec1/Ndc80 Tail Domain Function at the Kinetochore-Microtubule Interface.Frontiers in cell and developmental biology · 2020Review
- The Opposing Functions of Protein Kinases and Phosphatases in Chromosome Bipolar Attachment.International journal of molecular sciences · 2019Review
- Cell cycle-dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast.Molecular biology of the cell · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The interaction between chromosomes and spindle microtubules is essential for chromosome segregation. The kinetochore complex mediates this interaction. Previous studies indicate that the stability of kinetochore attachment is regulated by Aurora B/Ipl1 kinase and this regulation is conserved from yeast to mammalian cells. In budding yeast Saccharomyces cerevisiae, the ten-subunit Dam1/DASH complex bridges the interaction between kinetochores and microtubules, and some in vitro evidence indicates that the phosphorylation of Dam1 protein by Ipl1 kinase destabilizes this interaction. However, it is not clear if Dam1 phosphorylation is sufficient to regulate the stability of kinetochore attachment in vivo. Also, the significance of this regulation in response to chromosome detachment has not been fully investigated. Here we report that phospho-deficient dam1-3A mutants show stabilized kinetochore-microtubule attachment in vivo. This significantly delays the establishment of chromosome bipolar attachment after the disruption of kinetochore-microtubule interaction by a microtubule depolymerizing drug nocodazole. Moreover, dam1-3A cells show dramatic chromosome mis-segregation after treatment with nocodazole, presumably due to the combination of compromised bipolar attachment and premature spindle assembly checkpoint silencing in the mutant cells. Therefore, the regulation of Dam1 phosphorylation imposed by Ipl1 kinase is critical for faithful chromosome segregation.
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