ArticleEmerging microbes & infections2017
Cultivation of Anaplasma ovis in the HL-60 human promyelocytic leukemia cell line.
Article in Emerging microbes & infections, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- A novel phytopathogenFrontiers in cellular and infection microbiology · 2023Article
- The Scenario of Ticks and Tick-Borne Pathogens of Sheep on a Mediterranean Island.Microorganisms · 2022Article
- The first detection ofEmerging microbes & infections · 2021Article
- The Novel Zoonotic Pathogen,Pathogens (Basel, Switzerland) · 2021Article
- Identification and evaluation of UL36 protein from Dermacentor silvarum salivary gland and its interaction with Anaplasma ovis VirB10.Parasites & vectors · 2020Article
- A Multiplex PCR Detection Assay for the Identification of Clinically RelevantFrontiers in microbiology · 2020Article
- Dogs as New Hosts for the Emerging Zoonotic PathogenFrontiers in cellular and infection microbiology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tick-borne bacterium Anaplasma ovis is a widely distributed pathogen affecting sheep, goats and wild ruminants. Here, the HL-60 human promyelocytic leukemia cell line was used to isolate A. ovis from PCR-positive sheep and goats in Heilongjiang Province, China. Two weeks after inoculation, morulae were observed in cytoplasmic vacuoles in four different HL-60 cultures. Confocal microscopy using a Cy3-labeled A. ovis-specific probe confirmed that the HL-60 cells were infected with A. ovis. Cells from the 6th HL-60 subculture displayed positive fluorescence when incubated with A. ovis antiserum in the indirect fluorescent antibody assay. PCR amplification and sequencing of 16S rRNA, groEL, gltA, msp2 and msp4 Anaplasma genes revealed that the four A. ovis culture isolates were identical. Phylogenetic analysis showed that the sequences clustered with other A. ovis strains but could clearly be distinguished from other Anaplasma species. When the 18th subculture of infected HL-60 cells was examined by electron microscopy, lysosomes were often observed near the vacuoles. After the 24th subculture, Giemsa staining and PCR indicated that the HL-60 cells were negative for A. ovis. Although A. ovis can infect HL-60 cells for only four months, the ability of the organism to infect and multiply in HL-60 cells provides a tool to study intra-erythrocytic Anaplasma and host cell interactions.
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Registered trials
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