Evidence map›Paper›PMID 28924672›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT.

Brian J Beliveau, Alistair N Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang, C-Ting Wu

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.

0numbers the graph read from it
0cells of the map it votes in
77citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

77 citing papers in PubMed.

  1. Review
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  14. Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  15. Article
  16. From Biophysics to Biomedical Physics.ACS bio & med chem Au · 2025
    Review
  17. Article
  18. Article
  19. Review
  20. Tracing the Chromatin: From 3C to Live-Cell Imaging.Chemical & biomedical imaging · 2024
    Review

17 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Brian J BeliveauDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Alistair N BoettigerHoward Hughes Medical Institute, Cambridge, MA, 02138, USA.
Guy NirDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Bogdan BintuHoward Hughes Medical Institute, Cambridge, MA, 02138, USA.
Peng YinWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. py@hms.harvard.edu.
Xiaowei ZhuangHoward Hughes Medical Institute, Cambridge, MA, 02138, USA. zhuang@chemistry.harvard.edu.
C-Ting WuDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA. twu@genetics.med.harvard.edu.

Funding

Center for Genomically Engineered OrgansRM1HG008525 · NHGRI · HARVARD MEDICAL SCHOOL · PI CHURCH, GEORGE M · 2015 to 2019
$10.2M
Culling the human genome of disease variants using ultraconserved elementsR01HD091797 · NICHD · HARVARD MEDICAL SCHOOL · PI WU, CHAO-TING · 2016 to 2020
$5.5M
The inheritance of position: It's not just who you are, it's where you areDP1GM106412 · NIGMS · HARVARD MEDICAL SCHOOL · PI WU, CHAO-TING · 2012 to 2016
$4.2M
Super-resolution imaging via programmable autonomous blinkingR01EB018659 · NIBIB · HARVARD MEDICAL SCHOOL · PI YIN, PENG · 2013 to 2017
$3.3M
Ultra-Multiplexed Nanoscale In Situ Proteomics for Understanding Synapse TypesU01MH106011 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BATHE, MARK, BOYDEN, EDWARD S. · 2014 to 2016
$2.2M
Transvection and somatic homolog pairing in DrosophilaR01GM085169 · NIGMS · HARVARD MEDICAL SCHOOL · PI WU, CHAO-TING · 2009 to 2012
$2.1M
Single-molecule studies of ATP-dependent chromatin remodelingR01GM105637 · NIGMS · HARVARD UNIVERSITY · PI ZHUANG, XIAOWEI · 2013 to 2016
$1.3M
Howard Hughes Medical InstituteNHGRI NIH HHS RM1 HG008525NIBIB NIH HHS R01 EB018659NICHD NIH HHS R01 HD091797NIGMS NIH HHS DP1 GM106412NIGMS NIH HHS R01 GM085169NIGMS NIH HHS R01 GM105637NIMH NIH HHS U01 MH106011
6 · The paper itself

Abstract

OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila).

Indexed as

AnimalsDNADrosophilaGenomeHumansIn Situ Hybridization, FluorescenceMiceSingle Molecule ImagingDNAChromatinChromosomesDNA-PAINTFISHGenomeOligoDNA-PAINTOligopaintOligoSTORMSingle-moleculeSTORMSuper-resolution

Identifiers

PMID28924672
PMCPMC5919218

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.