ArticleApplications in plant sciences2017
Identification and characterization of microsatellite loci in the tuliptree,
Article in Applications in plant sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
premise of the studyTwenty-three polymorphic microsatellite loci (simple sequence repeats) were identified and characterized for METHODS AND
resultsUsing Illumina HiSeq paired-end reads from genomic DNA, searches for repeat motifs identified approximately 280,000 potentially amplifiable loci. Of 77 loci tested, 51 amplified consistently. When genotyped using 30 to 52 total adult trees from three old-growth populations in Maryland, Virginia, and New Jersey, USA, 23 loci were polymorphic. These loci exhibited four to 13 alleles, and observed and expected heterozygosities ranged from 0.233 to 0.865 and 0.272 to 0.876, respectively.
conclusionsThe microsatellite marker loci presented here will be valuable in population genetic studies of
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