ArticleApplications in plant sciences2017
Barcoded NS31/AML2 primers for sequencing of arbuscular mycorrhizal communities in environmental samples.
Article in Applications in plant sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Maximum entropy networks show that plant-arbuscular mycorrhizal fungi associations are anti-nested and modular.The New phytologist · 2026Article
- Invasive plants decrease arbuscular mycorrhizal fungal diversity and promote generalist fungal partners.The New phytologist · 2025Article
- Mycorrhizal Fungi Influence on Mature Tree Growth: Stronger in High-Nitrogen Soils for an EMF-Associated Tree and in Low-Nitrogen Soils for Two AMF-Associated Trees.Plant-environment interactions (Hoboken, N.J.) · 2025Article
- Catch crop amendments and microbial inoculants differently modulate apple rhizosphere microbiomes and plant responses.FEMS microbiology ecology · 2025Article
- Within-Site Variations in Soil Physicochemical Properties Explained the Spatiality and Cohabitation of Arbuscular Mycorrhizal Fungi in the Roots of Cryptomeria Japonica.Microbial ecology · 2024Article
- Arbuscular mycorrhizal diversity increases across a plant productivity gradient driven by soil nitrogen availability.Plant-environment interactions (Hoboken, N.J.) · 2024Article
- Effect of plant communities on bacterial and fungal communities in a Central European grassland.Environmental microbiome · 2024Article
- Article
- Diversity of Arbuscular Mycorrhizal Fungi in Distinct Ecosystems of the North Caucasus, a Temperate Biodiversity Hotspot.Journal of fungi (Basel, Switzerland) · 2023Article
- Ecological drivers of fine-scale distribution of arbuscular mycorrhizal fungi in a semiarid Mediterranean scrubland.Annals of botany · 2023Article
- Development of a taxon-discriminating molecular marker to trace and quantify a mycorrhizal inoculum in roots and soils of agroecosystems.Folia microbiologica · 2021Article
- A metabarcoding protocol targeting two DNA regions to analyze root-associated fungal communities in ferns and lycophytes.Applications in plant sciencesArticle
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2 authors.
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Abstract
premise of the studyArbuscular mycorrhizal fungi (AMF) are globally important root symbioses that enhance plant growth and nutrition and influence ecosystem structure and function. To better characterize levels of AMF diversity relevant to ecosystem function, deeper sequencing depth in environmental samples is needed. In this study, Illumina barcoded primers and a bioinformatics pipeline were developed and applied to study AMF diversity and community structure in environmental samples.
methodsLibraries of small subunit ribosomal RNA fragment amplicons were amplified from environmental DNA using a single-step PCR reaction with barcoded NS31/AML2 primers. Amplicons were sequenced on an Illumina MiSeq sequencer using version 2, 2 × 250-bp paired-end chemistry, and analyzed using QIIME and RDP Classifier.
resultsSequencing captured 196 to 6416 operational taxonomic units (OTUs; depending on clustering parameters) representing nine AMF genera. Regardless of clustering parameters, ∼20 OTUs dominated AMF communities (78-87% reads) with the remaining reads distributed among other OTUs. Analyses also showed significant biogeographic differences in AMF communities and that community composition could be linked to specific edaphic factors. DISCUSSION: Barcoded NS31/AML2 primers and Illumina MiSeq sequencing provide a powerful approach to address AMF diversity and variations in fungal assemblages across host plants, ecosystems, and responses to environmental drivers including global change.
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