ArticleScientific reports2017
Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: application to behavioral and developmental studies.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
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- Emerging tools to advance neuroethology in butterflies and moths.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- Cryptochrome-mediated integration of circadian rhythms into seasonal cycles in the silkwormFrontiers in physiology · 2026Review
- Genetic and viral approaches to record or manipulate neurons in insects.Current opinion in insect science · 2021Review
- An insulin-like growth factor-like peptide promotes ovarian development in the silkmoth Bombyx mori.Scientific reports · 2019Article
- Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system.Transgenic research · 2019Article
- Activity-Dependent Remodeling ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- An overview of functional genomic tools in deciphering insecticide resistance.Current opinion in insect science · 2018Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Silkmoth, Bombyx mori, is one of the important model insects in which transgenic techniques and the GAL4/UAS system are applicable. However, due to cytotoxicity and low transactivation activity of GAL4, effectiveness of the GAL4/UAS system and its application in B. mori are still limited. In the present study, we refined the previously reported UAS vector by exploiting transcriptional and translational enhancers, and achieved 200-fold enhancement of reporter GFP fluorescence in the GAL4/UAS system. Enhanced protein expression of membrane-targeted GFP and calcium indicator protein (GCaMP5G) drastically improved visualization of fine neurite structures and neural activity, respectively. Also, with the refined system, we generated a transgenic strain that expresses tetanus toxin light chain (TeTxLC), which blocks synaptic transmission, under the control of GAL4. Ectopic TeTxLC expression in the sex pheromone receptor neurons inhibited male courtship behavior, proving effectiveness of TeTxLC on loss-of-function analyses of neural circuits. In addition, suppression of prothoracicotropic hormone (PTTH) or insulin-like peptide (bombyxin) secretion impaired developmental timing and growth rate, respectively. Furthermore, we revealed that larval growth is sex-differentially regulated by these peptide hormones. The present study provides important technical underpinnings of transgenic approaches in silkmoths and insights into mechanisms of postembryonic development in insects.
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Registered trials
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