ArticleNature communications2017
Novel ecto-tagged integrins reveal their trafficking in live cells.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Who cites it
36 citing papers in PubMed, 61 citations in OpenAlex.
- Mechanical forces stimulate Golgi export.The Journal of cell biology · 2026Article
- Traction Force Microscopy with DNA FluoroCubes.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Integrins in cancer: insights into mechanisms and therapeutic potential.Cell communication and signaling : CCS · 2026Review
- Succinic Acid-Induced Macrophage Endocytosis Promotes Extracellular Vesicle-Based Integrin Beta1 Transfer Accelerating Fibroblast Activation and Sepsis-Associated Pulmonary Fibrosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Mapping Leak Electron Pathways from Mitochondria to the Outer Cell Membrane in Human Embryonic Lung Fibroblasts.Bioelectricity · 2025Article
- The kinesin KIF3AC recycles endocytosed integrin to polarize new adhesion formation toward the leading edge.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Sweet and sticky: increased cell adhesion through click-mediated functionalization of regenerative liver progenitor cells.Communications biology · 2025Article
- Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.The Journal of cell biology · 2025Article
- MYO18B promotes lysosomal exocytosis by facilitating focal adhesion maturation.The Journal of cell biology · 2025Article
- Luminescent sensing of conformational integrin activation in living cells.Cell reports · 2025Article
- Cell adhesion and spreading on fluid membranes through microtubules-dependent mechanotransduction.Nature communications · 2025Article
- Integrin signalling in joint development, homeostasis and osteoarthritis.Nature reviews. Rheumatology · 2024Review
- Sorting of secretory proteins at theeLife · 2024Article
- Fibroblasts generate topographical cues that steer cancer cell migration.Science advances · 2023Article
- Article
- Recruitment of the SNX17-Retriever recycling pathway regulates synaptic function and plasticity.The Journal of cell biology · 2023Article
- Article
- Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II-dependent fashion.The Journal of cell biology · 2023Article
- Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions.Nature reviews. Molecular cell biology · 2023Review
- Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis.Methods in molecular biology (Clifton, N.J.) · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Integrins are abundant heterodimeric cell-surface adhesion receptors essential in multicellular organisms. Integrin function is dynamically modulated by endo-exocytic trafficking, however, major mysteries remain about where, when, and how this occurs in living cells. To address this, here we report the generation of functional recombinant β1 integrins with traceable tags inserted in an extracellular loop. We demonstrate that these 'ecto-tagged' integrins are cell-surface expressed, localize to adhesions, exhibit normal integrin activation, and restore adhesion in β1 integrin knockout fibroblasts. Importantly, β1 integrins containing an extracellular pH-sensitive pHluorin tag allow direct visualization of integrin exocytosis in live cells and revealed targeted delivery of integrin vesicles to focal adhesions. Further, using β1 integrins containing a HaloTag in combination with membrane-permeant and -impermeant Halo dyes allows imaging of integrin endocytosis and recycling. Thus, ecto-tagged integrins provide novel powerful tools to characterize integrin function and trafficking.Integrins are cell-surface adhesion receptors that are modulated by endo-exocytic trafficking, but existing tools to study this process can interfere with function. Here the authors develop β1 integrins carrying traceable tags in the extracellular domain; a pH-sensitive pHlourin tag or a HaloTag to facilitate dye attachment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.