ArticleNature communications2017
SRpHi ratiometric pH biosensors for super-resolution microscopy.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
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- Addressing Particle Compositional Heterogeneities in Super-Resolution-Enhanced Live-Cell Ratiometric pH Sensing with Ultrasmall Fluorescent Core-Shell Aluminosilicate Nanoparticles.Advanced functional materials · 2021Article
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- Seeking Illumination: The Path to Chemiluminescent 1,2-Dioxetanes for Quantitative Measurements andAccounts of chemical research · 2021Article
- Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.Autophagy · 2021Article
- Clicking of organelle-enriched probes for fluorogenic imaging of autophagic and endocytic fluxes.Chemical science · 2021Article
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- TISSUE CLEARING.Nature reviews. Methods primers · 2021Article
- Upconversion nanoparticles as intracellular pH messengers.Analytical and bioanalytical chemistry · 2020Article
- Plasmonic Metasensors Based on 2D Hybrid Atomically Thin Perovskite Nanomaterials.Nanomaterials (Basel, Switzerland) · 2020Article
- Endosome motility defects revealed at super-resolution in live cells using HIDE probes.Nature chemical biology · 2020Article
- Optical Detection of Intracellular Quantities Using Nanoscale Technologies.Accounts of chemical research · 2019Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.