Evidence map›Paper›PMID 28923936›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2017

Isolation of exosomes from whole blood by integrating acoustics and microfluidics.

Mengxi Wu, Yingshi Ouyang, Zeyu Wang, Rui Zhang, Po-Hsun Huang, Chuyi Chen, Hui Li, Peng Li, David Quinn, Ming Dao and 3 more

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 425 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
425citing papers in PubMed, 1 pooled it
28.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

425 citing papers in PubMed, 1 synthesis or guideline pooled it, 908 citations in OpenAlex.

  1. Pooled it
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  17. Acoustofluidic Biosensors.Micromachines · 2026
    Review
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365 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 3 countries.

Mengxi WuDepartment of Mechanical Engineering and Material Science, Duke University, Durham, NC 27708.
Yingshi OuyangDepartment of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, PA 15213.
Zeyu WangDepartment of Mechanical Engineering and Material Science, Duke University, Durham, NC 27708.
Rui ZhangDepartment of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802.
Po-Hsun HuangDepartment of Mechanical Engineering and Material Science, Duke University, Durham, NC 27708.
Chuyi ChenDepartment of Mechanical Engineering and Material Science, Duke University, Durham, NC 27708.
Hui LiDepartment of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, PA 15213.
Peng LiEugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506.
David QuinnDepartment of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213.
Ming DaoDepartment of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; mingdao@mit.edu ssuresh@ntu.edu.sg ysadovsky@mwri.magee.edu tony.huang@duke.edu.
Subra SureshNanyang Technological University, Singapore 639798; mingdao@mit.edu ssuresh@ntu.edu.sg ysadovsky@mwri.magee.edu tony.huang@duke.edu.
Yoel SadovskyDepartment of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, PA 15213; mingdao@mit.edu ssuresh@ntu.edu.sg ysadovsky@mwri.magee.edu tony.huang@duke.edu.ORCID 0000-0003-2969-6737
Tony Jun HuangDepartment of Mechanical Engineering and Material Science, Duke University, Durham, NC 27708; mingdao@mit.edu ssuresh@ntu.edu.sg ysadovsky@mwri.magee.edu tony.huang@duke.edu.ORCID 0000-0003-1205-3313
Duke University · USUniversity of Pittsburgh · USPennsylvania State University · USCarnegie Mellon University · USCentral South University · CNMassachusetts Institute of Technology · USWest Virginia University · US

Funding

Extracellular vesicles and their ncRNA cargo as markers of trophoblast injuryR01HD086325 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI SADOVSKY, YOEL · 2015 to 2019
$3.6M
NICHD NIH HHS R01 HD086325
6 · The paper itself

Abstract

Exosomes are nanoscale extracellular vesicles that play an important role in many biological processes, including intercellular communications, antigen presentation, and the transport of proteins, RNA, and other molecules. Recently there has been significant interest in exosome-related fundamental research, seeking new exosome-based biomarkers for health monitoring and disease diagnoses. Here, we report a separation method based on acoustofluidics (i.e., the integration of acoustics and microfluidics) to isolate exosomes directly from whole blood in a label-free and contact-free manner. This acoustofluidic platform consists of two modules: a microscale cell-removal module that first removes larger blood components, followed by extracellular vesicle subgroup separation in the exosome-isolation module. In the cell-removal module, we demonstrate the isolation of 110-nm particles from a mixture of micro- and nanosized particles with a yield greater than 99%. In the exosome-isolation module, we isolate exosomes from an extracellular vesicle mixture with a purity of 98.4%. Integrating the two acoustofluidic modules onto a single chip, we isolated exosomes from whole blood with a blood cell removal rate of over 99.999%. With its ability to perform rapid, biocompatible, label-free, contact-free, and continuous-flow exosome isolation, the integrated acoustofluidic device offers a unique approach to investigate the role of exosomes in the onset and progression of human diseases with potential applications in health monitoring, medical diagnosis, targeted drug delivery, and personalized medicine.

Indexed as

AcousticsBlood CellsExosomesMicrofluidic Analytical Techniquesacoustic tweezersblood-borne vesiclesexosomesextracellular vesiclessurface acoustic waves

Identifiers

PMID28923936
PMCPMC5635903
OpenAlexW2754283165

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.