ArticleLipids in health and disease2017
Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application.
Article in Lipids in health and disease, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 85 citations in OpenAlex.
- Terrestrial bacterial isolate capability of producing surface-active biosurfactant: genetic identification, biochemical and morphological approach.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Activity evaluation and composition analysis of biosurfactants produced by an obligate cold-adapted bacterium Pedobacter sp. NJ-S-72 isolated from Antarctica.World journal of microbiology & biotechnology · 2026Article
- Brucella pituitosa strain BU72, a new hydrocarbonoclastic bacterium through exopolysaccharide-based surfactant production.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- A lipase from Lacticaseibacillus rhamnosus IDCC 3201 with thermostability and pH resistance for use as a detergent additive.Applied microbiology and biotechnology · 2024Article
- Molecular Cloning of the Extracellular Lipases ofIranian journal of biotechnology · 2024Article
- Co-production of lipases and biosurfactants by3 Biotech · 2024Article
- Production and Characterization of New Biosurfactants/Bioemulsifiers fromMolecules (Basel, Switzerland) · 2023Article
- Biotechnological potential of microbial bio-surfactants, their significance, and diverse applications.FEMS microbes · 2023Review
- Production and characterization of bioemulsifier byFrontiers in microbiology · 2023Article
- A comparative study on chemical characterization and properties of surface active compounds from Gram-positive Bacillus and Gram-negative Ochrobactrum strains utilizing pure hydrocarbons and waste mineral lubricating oils.World journal of microbiology & biotechnology · 2022Article
- Isolation, screening and molecular characterization of biosurfactant producing bacteria from soil samples of auto repair shops.Archives of microbiology · 2021Article
- The Effects of Food on Cannabidiol Bioaccessibility.Molecules (Basel, Switzerland) · 2021Article
- Microbial lipases and their industrial applications: a comprehensive review.Microbial cell factories · 2020Review
- Extreme environments: a source of biosurfactants for biotechnological applications.Extremophiles : life under extreme conditions · 2020Review
- Genome-Guided Characterization ofInternational journal of environmental research and public health · 2018Article
- Realm of Thermoalkaline Lipases in Bioprocess Commodities.Journal of lipids · 2018Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlkaline thermostable lipase and biosurfactant producing bacteria are very interested at detergent applications, not only because of their eco-friendly characterize, but alsoproduction lipase and biosurfactant by using cheap materials. Ochrobactrum intermedium strain MZV101 was isolated as washing powder resistant, alkaline thermostable lipase and biosurfactant producing bacterium in order to use at detergent applications.
methodsO. intermedium strain MZV101 produces was lipase and biosurfactant in the same media with pH 10 and temperature of 60 °C. Washing test and some detergent compatibility character of lipase enzyme and biosurfactant were assayed. The antimicrobial activity evaluated against various bacteria and fungi.
resultsLipase and biosurfactant produced by O. intermedium strain MZV101 exhibited high stability at pH 10-13 and temperature of 70-90 °C, biosurfactant exhibits good stability at pH 9-13 and thermostability in all range. Both lipase and biosurfactant were found to be stable in the presence of different metal ions, detergents and organic solvents. The lipase enzyme extracted using isopropanol with yield of 69.2% and biosurfactant with ethanol emulsification index value of 70.99% and yield of 9.32 (g/l). The single band protein after through from G-50 Sephadex column on SDS-PAGE was calculated to be 99.42 kDa. Biosurfactant O. intermedium strain MZV101 exhibited good antimicrobial activity against Gram-negative bacteria and against various bacterial pathogens. Based upon washing test biosurfactant and lipase O. intermedium strain MZV101considered being strong oil removal.
conclusionThe results of this study indicate that isolated lipase and biosurfactant with strong oil removal, antimicrobial activity and good stability could be useful for detergent applications.
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