ArticleACS synthetic biology2018
Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids.
Article in ACS synthetic biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Bioorthogonal chemistry for deciphering lipid biology in living systems.RSC chemical biology · 2026Review
- ΔmSystems · 2026Article
- Bacterial lipid synthesizing enzymes PlsY and PlsC utilize both stereo-forms of glycerol-phosphate.EMBO reports · 2026Article
- Breaking the membrane heredity paradox through de novo protocell formation.Nature communications · 2026Article
- A synthetic cell with integrated DNA self-replication and lipid biosynthesis.Nature communications · 2026Article
- Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells.FEBS letters · 2025Review
- Photochemical synthesis of natural lipids in artificial and living cells.Nature communications · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Biomimetic Materials to Fabricate Artificial Cells.Chemical reviews · 2024Review
- Synthetic Vesicles for Sustainable Energy Recycling and Delivery of Building Blocks for Lipid BiosynthesisACS synthetic biology · 2024Article
- Omega-3 and -6 Fatty Acids Alter the Membrane Lipid Composition and Vesicle Size to Regulate Exocytosis and Storage of Catecholamines.ACS chemical neuroscience · 2024Article
- SecYEG-mediated translocation in a model synthetic cell.Synthetic biology (Oxford, England) · 2024Article
- Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.ACS synthetic biology · 2023Review
- Synthesising a minimal cell with artificial metabolic pathways.Communications chemistry · 2023Article
- Exploring the Lipid World Hypothesis: A Novel Scenario of Self-Sustained Darwinian Evolution of the Liposomes.Astrobiology · 2023Article
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis.ACS synthetic biology · 2022Article
- Synthesis of lipid membranes for artificial cells.Nature reviews. Chemistry · 2021Review
- Toward synthetic life: Biomimetic synthetic cell communication.Current opinion in chemical biology · 2021Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple fatty acid building blocks and glycerol 3-phosphate. The reconstituted system yields multiple phospholipid species that vary in acyl-chain and polar headgroup compositions. Due to the high fidelity and versatility, complete conversion of the fatty acid substrates into multiple phospholipid species is achieved simultaneously, leading to membrane expansion as a first step toward a synthetic minimal cell.
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