ArticleGigaScience2017
De novo genome assembly of Camptotheca acuminata, a natural source of the anti-cancer compound camptothecin.
Article in GigaScience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 97 citations in OpenAlex.
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- DIMORPH: an integrated multi-omics resource for camptothecin-producing plants.Molecular horticulture · 2026Article
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- Natural Alkaloids as Antimicrobial Agents: Mechanisms, Potentials and Challenges.Molecules (Basel, Switzerland) · 2026Review
- Orthologue inference-based enzyme mining for diversification of the anti-cancer evodiamine scaffold.Communications chemistry · 2026Article
- Unravelling DNA methylation dynamics during developmental stages in Quercus ilex subsp. ballota [Desf.] Samp.BMC plant biology · 2024Article
- Transcriptome and metabolome analysis reveals mechanism of light intensity modulating iridoid biosynthesis in Gentiana macrophylla Pall.BMC plant biology · 2024Article
- Phytocannabinoids CBD, CBG, and their Derivatives CBD-HQ and CBG-A Induced In Vitro Cytotoxicity in 2D and 3D Colon Cancer Cell Models.Current issues in molecular biology · 2024Article
- Insights into Metabolic Engineering of Bioactive Molecules inCurrent genomics · 2023Review
- Light regulation of the biosynthesis of phenolics, terpenoids, and alkaloids in plants.Communications biology · 2023Review
- Genomic and transcriptomic analysis of camptothecin producing novel fungal endophyte: Alternaria burnsii NCIM 1409.Scientific reports · 2023Article
- Genome-scale metabolic model led engineering ofFrontiers in plant science · 2023Article
- A first draft genome of holm oak (Frontiers in molecular biosciences · 2023Article
- Concept of Hybrid Drugs and Recent Advancements in Anticancer Hybrids.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Functional characterization of a catalytically promiscuous tryptophan decarboxylase from camptothecin-producingFrontiers in plant science · 2022Article
- Plants in Anticancer Drug Discovery: From Molecular Mechanism to Chemoprevention.BioMed research international · 2022Review
- Integrative Analysis of Elicitor-Induced Camptothecin Biosynthesis inFrontiers in plant science · 2022Article
- Cannabidiol Effectively Promoted Cell Death in Bladder Cancer and the Improved Intravesical Adhesion Drugs Delivery Strategy Could Be Better Used for Treatment.Pharmaceutics · 2021Article
- TCM-Blast for traditional Chinese medicine genome alignment with integrated resources.BMC plant biology · 2021Article
- Divergent camptothecin biosynthetic pathway in Ophiorrhiza pumila.BMC biology · 2021Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Camptotheca acuminata is 1 of a limited number of species that produce camptothecin, a pentacyclic quinoline alkaloid with anti-cancer activity due to its ability to inhibit DNA topoisomerase. While transcriptome studies have been performed previously with various camptothecin-producing species, no genome sequence for a camptothecin-producing species is available to date. We generated a high-quality de novo genome assembly for C. acuminata representing 403 174 860 bp on 1394 scaffolds with an N50 scaffold size of 1752 kbp. Quality assessments of the assembly revealed robust representation of the genome sequence including genic regions. Using a novel genome annotation method, we annotated 31 825 genes encoding 40 332 gene models. Based on sequence identity and orthology with validated genes from Catharanthus roseus as well as Pfam searches, we identified candidate orthologs for genes potentially involved in camptothecin biosynthesis. Extensive gene duplication including tandem duplication was widespread in the C. acuminata genome, with 2571 genes belonging to 997 tandem duplicated gene clusters. To our knowledge, this is the first genome sequence for a camptothecin-producing species, and access to the C. acuminata genome will permit not only discovery of genes encoding the camptothecin biosynthetic pathway but also reagents that can be used for heterologous expression of camptothecin and camptothecin analogs with novel pharmaceutical applications.
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