ArticleJournal of experimental botany2017
A pentatricopeptide repeat protein restores nap cytoplasmic male sterility in Brassica napus.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 89 citations in OpenAlex.
- Mitochondrial gene orf224 triggers retrograde signaling and reprogramming of anther cell fate in Brassica napus Polima CMS.The New phytologist · 2026Article
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- A PPR gene cluster from Solanum aethiopicum underlies fertility restoration in cytoplasmic male-sterile eggplant (S. melongena L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- A major FT/TFL1 regulatory locus (Frontiers in plant science · 2026Article
- A pentatricopeptide repeat protein restores fertility in Moricandia arvensis based cytoplasmic male sterility system in Brassica juncea.Molecular genetics and genomics : MGG · 2025Article
- Identification and variation of a new restorer of fertility gene that induces cleavage in orf138 mRNA of Ogura male sterility in radish.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Commercial suitability and characterization of newly developed Erucastrum canariense (Can) sterile cytoplasm based cytoplasmic male sterile (CMS) lines in Indian cauliflower.Scientific reports · 2024Article
- Fine mapping and candidate gene analysis of a novel fertility restorer gene for C-type cytoplasmic male sterility in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Functional Differences of Grapevine Circular RNAPlants (Basel, Switzerland) · 2023Article
- Assembly and analysis of the mitochondrial genome ofFrontiers in plant science · 2023Article
- Development of mitochondrial simple sequence repeat markers to simultaneously distinguish cytoplasmic male sterile sources in cotton.Functional & integrative genomics · 2022Article
- Identification of a CandidateInternational journal of molecular sciences · 2022Article
- A super PPR cluster for restoring fertility revealed by genetic mapping, homocap-seq and de novo assembly in cotton.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Molecular Analysis Uncovers the Mechanism of Fertility Restoration in Temperature-Sensitive Polima Cytoplasmic Male-SterileInternational journal of molecular sciences · 2021Article
- Functions of PPR Proteins in Plant Growth and Development.International journal of molecular sciences · 2021Review
- The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research.G3 (Bethesda, Md.) · 2021Article
- A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- High-generation near-isogenic lines combined with multi-omics to study the mechanism of polima cytoplasmic male sterility.BMC plant biology · 2021Article
- Physical mapping and InDel marker development for the restorer gene RfBMC genomics · 2021Article
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two forms of male-sterile cytoplasm, designated nap and pol, are found in the oilseed rape species, Brassica napus. The nap cytoplasm is observed in most B. napus varieties, and it confers male sterility on a limited number of cultivars that lack the corresponding restorer gene, Rfn. In the present study, using linkage analysis in combination with 5652 BC1 progeny derived from a cross between a nap cytoplasmic male sterility (CMS) line 181A and a restorer line H5, we delimited the Rfn gene to a 10.5 kb region on chromosome A09, which contained three putative ORFs. Complementation by transformation rescue revealed that the introduction of ORF2, which encodes a pentatricopeptide repeat (PPR) protein, resulted in the recovery of fertility of nap CMS plants. Expression analysis suggested that the Rfn was highly expressed in flower buds and it was preferentially expressed in the tapetum and meiocytes during anther development. Further RNA gel blots and immunodetection suggested that the Rfn gene may play a complicated role in restoring the nap CMS. Our work laid the foundation for dissecting the molecular basis of CMS fertility restoration and the nuclear-mitochondrial interactions in CMS/Rf systems.
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