ArticleJournal of experimental botany2017
Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis-next generation sequencing and virus-induced gene silencing strategies.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Genetic Mapping and Functional Characterisation of GhCUP Regulating Leaf Curling in Cotton.Plant biotechnology journal · 2026Article
- The Aromatic Amino Acid Biosynthesis GeneMicroorganisms · 2025Article
- The Genetic Loci Associated with Fiber Development in Upland Cotton (Plants (Basel, Switzerland) · 2025Article
- Deletion of GhSCY2D Causes Impaired Chloroplast Development and Temperature-Dependent Leaf Yellowing in Cotton (Gossypium hirsutum L.).Plant, cell & environment · 2025Article
- A CC-NB-ARC-LRR Gene Regulates Bract Morphology in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- GHCU, a Molecular Chaperone, Regulates Leaf Curling by Modulating the Distribution of KNGH1 in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Identification of Clubroot (Genes · 2024Article
- Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding.Frontiers in plant science · 2024Review
- The genetic architecture of prolificacy in maize revealed by association mapping and bulk segregant analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Melon yellow-green plant (Cmygp) encodes a Golden2-like transcription factor regulating chlorophyll synthesis and chloroplast development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton.Frontiers in plant science · 2023Article
- Genetic mapping and molecular characterization of the delayed green geneFrontiers in plant science · 2023Article
- Comparative Genomics and Functional Studies of Putative mInternational journal of molecular sciences · 2022Article
- Fine-mapping and identification of a candidate gene controlling seed coat color in melon (Cucumis melo L. var. chinensis Pangalo).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- An autopolyploid-suitable polyBSA-seq strategy for screening candidate genetic markers linked to leaf blight resistance in sugarcane.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Article
- Genome sequencing identified novel mechanisms underlying virescent mutation in upland cotton Gossypiuma hirsutum.BMC genomics · 2021Article
- Genomic mapping and identification of candidate genes encoding nulliplex-branch trait in sea-island cotton (Molecular breeding : new strategies in plant improvement · 2021Article
- Rapid Mining of Candidate Genes for Verticillium Wilt Resistance in Cotton Based on BSA-Seq Analysis.Frontiers in plant science · 2021Article
- A high-density genetic map and multiple environmental tests reveal novel quantitative trait loci and candidate genes for fibre quality and yield in cotton.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
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9 authors.
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Abstract
Map-based gene cloning is a vital strategy for the identification of the quantitative trait loci or genes underlying important agronomic traits. The conventional map-based cloning method is powerful but generally time-consuming and labor-intensive. In this context, we introduce an improved bulked segregant analysis method in combination with a virus-induced gene silencing (VIGS) strategy for rapid and reliable gene mapping, identification and functional verification. This method was applied to a multiple recessive marker line of upland cotton, Texas 582 (T582), and identified unique genomic positions harboring mutant loci, showing the reliability and efficacy of this method. The v1 locus was further fine-mapped. Only one gene, GhCHLI, which encodes one of the subunits of Mg chelatase, was differentially down-regulated in T582 compared with TM-1. A point mutation occurred in the AAA+ conserved region of GhCHLI and led to an amino acid substitution. Suppression of its expression by VIGS in TM-1 resulted in a yellow blade phenotype that was similar to T582. This integrated approach provides a paradigm for the rapid mapping and identification of the candidate genes underlying the genetic traits in plants with large and complex genomes in the future.
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