Evidence map›Paper›PMID 28922568›Full record

ArticleProteomics2017

Spectral Libraries for SWATH-MS Assays for Drosophila melanogaster and Solanum lycopersicum.

Bertrand Fabre, Dagmara Korona, Clara I Mata, Harriet T Parsons, Michael J Deery, Maarten L A T M Hertog, Bart M Nicolaï, Steven Russell, Kathryn S Lilley

Abstract read
In one paragraph

Article in Proteomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. In Depth Exploration of the Alternative Proteome ofFrontiers in cell and developmental biology · 2022
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Bertrand FabreCambridge Centre for Proteomics Department of Biochemistry, University of Cambridge, Cambridge, UK.
Dagmara KoronaDepartment of Genetics University of Cambridge, Cambridge, UK.
Clara I MataBIOSYST-MeBioS, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0003-3432-839X
Harriet T ParsonsCambridge Centre for Proteomics Department of Biochemistry, University of Cambridge, Cambridge, UK.
Michael J DeeryCambridge Centre for Proteomics Department of Biochemistry, University of Cambridge, Cambridge, UK.
Maarten L A T M HertogBIOSYST-MeBioS, KU Leuven, Leuven, Belgium.
Bart M NicolaïBIOSYST-MeBioS, KU Leuven, Leuven, Belgium.
Steven RussellDepartment of Genetics University of Cambridge, Cambridge, UK.
Kathryn S LilleyCambridge Centre for Proteomics Department of Biochemistry, University of Cambridge, Cambridge, UK.

Funding

Biotechnology and Biological Sciences Research Council BB/L002817/1
6 · The paper itself

Abstract

Quantitative proteomics methods have emerged as powerful tools for measuring protein expression changes at the proteome level. Using MS-based approaches, it is now possible to routinely quantify thousands of proteins. However, prefractionation of the samples at the protein or peptide level is usually necessary to go deep into the proteome, increasing both MS analysis time and technical variability. Recently, a new MS acquisition method named SWATH is introduced with the potential to provide good coverage of the proteome as well as a good measurement precision without prior sample fractionation. In contrast to shotgun-based MS however, a library containing experimental acquired spectra is necessary for the bioinformatics analysis of SWATH data. In this study, spectral libraries for two widely used models are built to study crop ripening or animal embryogenesis, Solanum lycopersicum (tomato) and Drosophila melanogaster, respectively. The spectral libraries comprise fragments for 5197 and 6040 proteins for S. lycopersicum and D. melanogaster, respectively, and allow reproducible quantification for thousands of peptides per MS analysis. The spectral libraries and all MS data are available in the MassIVE repository with the dataset identifiers MSV000081074 and MSV000081075 and the PRIDE repository with the dataset identifiers PXD006493 and PXD006495.

Indexed as

AnimalsDrosophila melanogasterPeptide LibraryProteomeProteomicsReference StandardsSolanum lycopersicumTandem Mass SpectrometryPeptide LibraryProteomeDrosophila melanogasterSolanum lycopersicumspectral librarySWATH

Identifiers

PMID28922568
PMCPMC5821221

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.