Evidence map›Paper›PMID 28921456›Full record

ArticleMolecular neurobiology2018

Blood-Brain Barrier in a Haemophilus influenzae Type a In Vitro Infection: Role of Adenosine Receptors A

N Caporarello, M Olivieri, M Cristaldi, M Scalia, M A Toscano, C Genovese, A Addamo, M Salmeri, G Lupo, C D Anfuso

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 47 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Blood-Brain Barrier Breakdown in Neuroinflammation: Current In Vitro Models.International journal of molecular sciences · 2023
    Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Antimicrobial, Antioxidant, and Cytotoxic Activities ofAntibiotics (Basel, Switzerland) · 2021
    Article
  17. Purinergic Regulation of Endothelial Barrier Function.International journal of molecular sciences · 2021
    Review
  18. Article
  19. Frontiers in microbiology · 2021
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

N CaporarelloSection of Medical Biochemistry, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
M OlivieriSection of Medical Biochemistry, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
M CristaldiSection of Medical Biochemistry, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
M ScaliaSection of Biology and Genetic, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
M A ToscanoSection of Microbiology, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
C GenoveseSection of Microbiology, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
A AddamoSection of Microbiology, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
M SalmeriSection of Microbiology, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy. msalmeri@unict.it.
G LupoSection of Medical Biochemistry, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy. gabriella.lupo@unict.it.ORCID http://orcid.org/0000-0002-9291-6969
C D AnfusoSection of Medical Biochemistry, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
University of Catania · IT

Funding

National Grant PON01- 00110
6 · The paper itself

Abstract

The blood-brain barrier (BBB) is mainly made up of tightly connected microvascular endothelial cells (BMECs), surrounded by pericytes (BMPCs) which regulate BBB tightness by providing soluble factors that control endothelial proliferation. Haemophilus influenzae type a (Hia) is able to reach the BBB, crossing it, thus causing meningitis. In this study, by using an in vitro model of BBB, performed with human BMECs and human BMPCs in co-culture, we demonstrated that, after Hia infection, the number of hBMPCs decreased whereas the number of hBMECs increased in comparison with non-infected cells. SEM and TEM images showed that Hia was able to enter hBMECs and reduce TEER and VE-cadherin expression. When the cells were infected in presence of SCH58261 and PSB603 but not DPCPX, an increase in TEER values was observed thus demonstrating that A

Indexed as

Blood-Brain BarrierCadherinsCell CountCoculture TechniquesCyclic AMPCyclic AMP Response Element-Binding ProteinElectric ImpedanceEndothelial CellsHaemophilus InfectionsHaemophilus influenzaeHumansMicrovesselsPericytesPhosphorylationReceptor, Adenosine A2AReceptor, Adenosine A2BCadherinsCyclic AMPCyclic AMP Response Element-Binding ProteinReceptor, Adenosine A2AReceptor, Adenosine A2Brho GTP-Binding ProteinsVascular Endothelial Growth Factor AAdenosine receptorsBacterial infectionBlood–brain barrierCo-culturesHuman brain microvascular endothelial cellsHuman brain microvascular pericytesVEGF

Identifiers

PMID28921456
OpenAlexW2755745544

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.