Evidence map›Paper›PMID 28917051›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Selenocysteine-Mediated Expressed Protein Ligation of SELENOM.

Jun Liu, Qingqing Chen, Sharon Rozovsky

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jun LiuDepartment of Chemistry and Biochemistry, University of Delaware, 136 Brown Laboratory, Newark, DE, 19716, USA.
Qingqing ChenDepartment of Chemistry and Biochemistry, University of Delaware, 136 Brown Laboratory, Newark, DE, 19716, USA.
Sharon RozovskyDepartment of Chemistry and Biochemistry, University of Delaware, 136 Brown Laboratory, Newark, DE, 19716, USA. rozovsky@udel.edu.
University of Delaware · US

Funding

This renovation project will create over 1455 sq. ft. of state- of-the-art reseaP20GM104316 · NIGMS · UNIVERSITY OF DELAWARE · PI SCHMITZ, KARL ROBERT · 2014 to 2024
$26.8M
Pilot Research Subproject ProgramP30GM110758 · NIGMS · UNIVERSITY OF DELAWARE · PI PATEL, SANDEEP · 2014 to 2018
$5.8M
CHEMISTRY-BIOLOGY INTERFACE PREDOCTORAL TRAININGT32GM008550 · NIGMS · UNIVERSITY OF DELAWARE · PI BAHNSON, BRIAN J · 1994 to 2018
$5.0M
Chemistry-Biology Interface Predoctoral Training Grant 2024-2029T32GM133395 · NIGMS · UNIVERSITY OF DELAWARE · PI Catherine Leimkuhler Grimes · 2019 to 2026
$3.7M
NIGMS NIH HHS P20 GM104316NIGMS NIH HHS P30 GM110758NIGMS NIH HHS T32 GM008550NIGMS NIH HHS T32 GM133395
6 · The paper itself

Abstract

A sizeable fraction of the selenoproteome encodes oxidoreductases possessing a thioredoxin fold, a structural motif that is shared among a diverse group of enzymes. In these oxidoreductases, the active site is comprised of a cysteine and a selenocysteine separated by one to two amino acids. In a subset of these selenoproteins, such as human SELENOH, SELENOM, SELENOT, SELENOV, SELENOW, and SELENOF, this redox motif is positioned immediately after the first β-sheet in a short loop, and is essential for interactions with its substrate or partners. Here, we describe the preparation of a representative member of this group, SELENOM, by selenocysteine-driven expressed protein ligation. The preparation employs a peptide bond formation between two protein fragments expressed recombinantly in E. coli. This method can be employed to prepare other selenoproteins.

Indexed as

Gene ExpressionRecombinant Fusion ProteinsCircular DichroismCloning, MolecularMass SpectrometryModels, MolecularPlasmidsProtein ConformationSelenocysteineSelenoproteinsRecombinant Fusion ProteinsSelenocysteineSelenoproteinsExpressed protein ligationSelenocysteine-mediated expressed protein ligationSELENOMSelenoprotein MSelenoproteins

Identifiers

PMID28917051
PMCPMC5821492
OpenAlexW2755771691

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.