Evidence map›Paper›PMID 28916981›Full record

ArticleTransgenic research2017

Expression of a novel bi-directional Brassica napus promoter in soybean.

Siva Chennareddy, Toby Cicak, Lauren Clark, Sean Russell, Michiyo Skokut, Jeffrey Beringer, Xiaozeng Yang, Yi Jia, Manju Gupta

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Siva ChennareddyDow AgroSciences, West Lafayette, IN, 47906, USA. schennareddy@dow.com.ORCID 0000-0001-8659-5399
Toby CicakDow AgroSciences, West Lafayette, IN, 47906, USA.
Lauren ClarkDow AgroSciences, Indianapolis, IN, 46268, USA.
Sean RussellDow AgroSciences, Indianapolis, IN, 46268, USA.
Michiyo SkokutDow AgroSciences, Indianapolis, IN, 46268, USA.
Jeffrey BeringerDow AgroSciences, Indianapolis, IN, 46268, USA.
Xiaozeng YangBeijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Yi JiaDow AgroSciences, Indianapolis, IN, 46268, USA.
Manju GuptaDow AgroSciences, Indianapolis, IN, 46268, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The expression profile of a natural bi-directional promoter, derived from the Brassica napus EPSPS-A gene, was studied in transgenic soybean (Glycine max C.V. Maverick) lines. Two constructs, pDAB100331 and pDAB100333, were assembled to test the bi-directionality of the promoter. Two reporter genes, gfp and gusA, were employed and they were interchangeably placed in both constructs, one on each end of the promoter such that both proteins expressed divergently in each construct. In the T

Indexed as

Plants, Genetically ModifiedPromoter Regions, Genetic3-Phosphoshikimate 1-Carboxyvinyltransferase5' Untranslated RegionsBrassica napusGene Expression Regulation, PlantGlycine maxGreen Fluorescent ProteinsPlant Proteins3-Phosphoshikimate 1-Carboxyvinyltransferase5' Untranslated RegionsGreen Fluorescent ProteinsPlant ProteinsAgrobacterium-mediated transformationBi-directional promoterGene expressionGFPGUSSoybean (Glycine max)

Identifiers

PMID28916981

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.