ArticleScientific reports2017
Time-resolved analysis of DNA-protein interactions in living cells by UV laser pulses.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- Two-dimensional nanostructures based '-onics' and '-omics' in personalized medicine.Nanophotonics (Berlin, Germany) · 2022Review
- Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study.Bioengineering (Basel, Switzerland) · 2022Article
- MicroRNA-550a-3-5p controls the brain metastasis of lung cancer by directly targeting YAP1.Cancer cell international · 2021Article
- Depletion of the DarG antitoxin in Mycobacterium tuberculosis triggers the DNA-damage response and leads to cell death.Molecular microbiology · 2020Article
- Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry.Nature communications · 2020Article
- In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure.Journal of visualized experiments : JoVE · 2019Article
- Cancer epigenetics: Moving forward.PLoS genetics · 2018Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interactions between DNA and proteins are mainly studied through chemical procedures involving bi-functional reagents, mostly formaldehyde. Chromatin immunoprecipitation is used to identify the binding between transcription factors (TFs) and chromatin, and to evaluate the occurrence and impact of histone/DNA modifications. The current bottleneck in probing DNA-protein interactions using these approaches is caused by the fact that chemical crosslinkers do not discriminate direct and indirect bindings or short-lived chromatin occupancy. Here, we describe a novel application of UV laser-induced (L-) crosslinking and demonstrate that a combination of chemical and L-crosslinking is able to distinguish between direct and indirect DNA-protein interactions in a small number of living cells. The spatial and temporal dynamics of TF bindings to chromatin and their role in gene expression regulation may thus be assessed. The combination of chemical and L-crosslinking offers an exciting and unprecedented tool for biomedical applications.
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Registered trials
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