ArticleScientific reports2017
Automated N-Glycosylation Sequencing Of Biopharmaceuticals By Capillary Electrophoresis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Operationalizing digital twins in biomanufacturing through interoperable process analytical technology.Bioprocess and biosystems engineering · 2026Article
- Digital microfluidics-engaged automated enzymatic degradation and synthesis of oligosaccharides.Frontiers in bioengineering and biotechnology · 2023Article
- A Rapid Protocol for Preparing 8-Aminopyrene-1,3,6-Trisulfonate-Labeled Glycans for Capillary Electrophoresis-Based Enzyme Assays.Methods in molecular biology (Clifton, N.J.) · 2023Article
- High-Throughput Glycomic Methods.Chemical reviews · 2022Review
- Introduction of a Capillary Gel Electrophoresis-Based Workflow for Biotherapeutics Characterization: Size, Charge, andFrontiers in bioengineering and biotechnology · 2022Article
- Article
- High-throughput N-glycan screening method for therapeutic antibodies using a microchip-based DNA analyzer: a promising methodology for monitoring monoclonal antibody N-glycosylation.Analytical and bioanalytical chemistry · 2021Article
- Capillary (Gel) Electrophoresis-Based Methods for Immunoglobulin (G) Glycosylation Analysis.Experientia supplementum (2012) · 2021Article
- Simplifying Glycan Profiling through a High-Throughput Micropermethylation Strategy.SLAS technology · 2020Article
- Structural and In Vitro Functional Comparability Analysis of Altebrel™, a Proposed Etanercept Biosimilar: Focus on Primary Sequence and Glycosylation.Pharmaceuticals (Basel, Switzerland) · 2019Article
- Capillary Electrophoresis Separations of Glycans.Chemical reviews · 2018Review
- Advances in enzyme substrate analysis with capillary electrophoresis.Methods (San Diego, Calif.) · 2018Review
- α2-6-Neosialidase: A Sialyltransferase Mutant as a Sialyl Linkage-Specific Sialidase.ACS chemical biology · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive analysis of the N-linked carbohydrates of glycoproteins is gaining high recent interest in both the biopharmaceutical and biomedical fields. In addition to high resolution glycosylation profiling, sugar residue and linkage specific enzymes are also routinely used for exoglycosidase digestion based carbohydrate sequencing. This latter one, albeit introduced decades ago, still mostly practiced by following tedious and time consuming manual processes. In this paper we introduce an automated carbohydrate sequencing approach using the appropriate exoglycosidase enzymes in conjunction with the utilization of some of the features of a capillary electrophoresis (CE) instrument to speed up the process. The enzymatic reactions were accomplished within the temperature controlled sample storage compartment of a capillary electrophoresis unit and the separation capillary was also utilized for accurate delivery of the exoglycosidase enzymes. CE analysis was conducted after each digestion step obtaining in this way the sequence information of N-glycans in 60 and 128 minutes using the semi- and the fully-automated methods, respectively.
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