ArticleThe Journal of biological chemistry2017
Glucose availability controls adipogenesis in mouse 3T3-L1 adipocytes via up-regulation of nicotinamide metabolism.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 49 citations in OpenAlex.
- RB1 controls differentiation through positive regulation of phosphoglycerate mutases.Cell death & disease · 2025Article
- Regulation of adipogenesis by nucleotides.Biochemical Society transactions · 2025Review
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- Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation.Cell stem cell · 2025Article
- Adipogenic Differentiation Assay for the Study of Insulin Signaling.Methods in molecular biology (Clifton, N.J.) · 2025Article
- PFKFB3 protein in adipose tissue contributes to whole body glucose homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis.Adipocyte · 2023Article
- Metallothionein 3 Inhibits 3T3-L1 Adipocyte Differentiation via Reduction of Reactive Oxygen Species.Antioxidants (Basel, Switzerland) · 2023Article
- Anti-obesity effects by parasitic nematode (Frontiers in cellular and infection microbiology · 2023Article
- Insulin prevents fatty acid induced increase of adipocyte size.Adipocyte · 2022Article
- Different Seasonal Collections ofNutrients · 2022Article
- High glutamine suppresses osteogenesis through mTORC1-mediated inhibition of the mTORC2/AKT-473/RUNX2 axis.Cell death discovery · 2022Article
- Article
- Weighing in on Adipogenesis.Frontiers in physiology · 2022Review
- Effect of Metformin and Simvastatin in Inhibiting Proadipogenic Transcription Factors.Current issues in molecular biology · 2021Article
- Article
- Adipokine Apelin/APJ Pathway Promotes Peritoneal Dissemination of Ovarian Cancer Cells by Regulating Lipid Metabolism.Molecular cancer research : MCR · 2021Article
- PFKFB3-dependent glucose metabolism regulates 3T3-L1 adipocyte development.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Chronic and Transient Hyperglycemia Induces Changes in the Expression Patterns ofInternational journal of molecular sciences · 2021Article
- NADP modulates RNA mNature chemical biology · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expansion of adipose tissue in response to a positive energy balance underlies obesity and occurs through both hypertrophy of existing cells and increased differentiation of adipocyte precursors (hyperplasia). To better understand the nutrient signals that promote adipocyte differentiation, we investigated the role of glucose availability in regulating adipocyte differentiation and maturation. 3T3-L1 preadipocytes were grown and differentiated in medium containing a standard differentiation hormone mixture and either 4 or 25 mm glucose. Adipocyte maturation at day 9 post-differentiation was determined by key adipocyte markers, including glucose transporter 4 (GLUT4) and adiponectin expression and Oil Red O staining of neutral lipids. We found that adipocyte differentiation and maturation required a pulse of 25 mm glucose only during the first 3 days of differentiation. Importantly, fatty acids were unable to substitute for the 25 mm glucose pulse during this period. The 25 mm glucose pulse increased adiponectin and GLUT4 expression and accumulation of neutral lipids via distinct mechanisms. Adiponectin expression and other early markers of differentiation required an increase in the intracellular pool of total NAD/P. In contrast, GLUT4 protein expression was only partially restored by increased NAD/P levels. Furthermore, GLUT4 mRNA expression was mediated by glucose-dependent activation of GLUT4 gene transcription through the cis-acting GLUT4-liver X receptor element (LXRE) promoter element. In summary, this study supports the conclusion that high glucose promotes adipocyte differentiation via distinct metabolic pathways and independently of fatty acids. This may partly explain the mechanism underlying adipocyte hyperplasia that occurs much later than adipocyte hypertrophy in the development of obesity.
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