Evidence map›Paper›PMID 28913866›Full record

ArticlePlant biotechnology journal2018

Conversion of array-based single nucleotide polymorphic markers for use in targeted genotyping by sequencing in hexaploid wheat (Triticum aestivum).

Amanda J Burridge, Paul A Wilkinson, Mark O Winfield, Gary L A Barker, Alexandra M Allen, Jane A Coghill, Christy Waterfall, Keith J Edwards

Abstract read
In one paragraph

Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. The Bra1K array: an efficient and versatile 1K liquid-phase SNP chip for genetics and breeding in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
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  18. Generation of Doubled Haploid Wheat-Frontiers in plant science · 2021
    Article
  19. Review
  20. International journal of molecular sciences · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Amanda J BurridgeLife Sciences, University of Bristol, Bristol, UK.
Paul A WilkinsonLife Sciences, University of Bristol, Bristol, UK.
Mark O WinfieldLife Sciences, University of Bristol, Bristol, UK.
Gary L A BarkerLife Sciences, University of Bristol, Bristol, UK.
Alexandra M AllenLife Sciences, University of Bristol, Bristol, UK.
Jane A CoghillLife Sciences, University of Bristol, Bristol, UK.
Christy WaterfallLife Sciences, University of Bristol, Bristol, UK.
Keith J EdwardsLife Sciences, University of Bristol, Bristol, UK.

Funding

Biotechnology and Biological Sciences Research Council BB/I003207/1Biotechnology and Biological Sciences Research Council BB/L01386X/1Biotechnology and Biological Sciences Research Council BB/N021061/1
6 · The paper itself

Abstract

Wheat breeders and academics alike use single nucleotide polymorphisms (SNPs) as molecular markers to characterize regions of interest within the hexaploid wheat genome. A number of SNP-based genotyping platforms are available, and their utility depends upon factors such as the available technologies, number of data points required, budgets and the technical expertise required. Unfortunately, markers can rarely be exchanged between existing and newly developed platforms, meaning that previously generated data cannot be compared, or combined, with more recently generated data sets. We predict that genotyping by sequencing will become the predominant genotyping technology within the next 5-10 years. With this in mind, to ensure that data generated from current genotyping platforms continues to be of use, we have designed and utilized SNP-based capture probes from several thousand existing and publicly available probes from Axiom® and KASP™ genotyping platforms. We have validated our capture probes in a targeted genotyping by sequencing protocol using 31 previously genotyped UK elite hexaploid wheat accessions. Data comparisons between targeted genotyping by sequencing, Axiom® array genotyping and KASP™ genotyping assays, identified a set of 3256 probes which reliably bring together targeted genotyping by sequencing data with the previously available marker data set. As such, these probes are likely to be of considerable value to the wheat community. The probe details, full probe sequences and a custom built analysis pipeline may be freely downloaded from the CerealsDB website (http://www.cerealsdb.uk.net/cerealgenomics/CerealsDB/sequence_capture.php).

Indexed as

Polymorphism, Single NucleotideDNA ProbesGenotyping TechniquesOligonucleotide Array Sequence AnalysisPolyploidyTriticumDNA Probesgenotyping by sequencingsingle nucleotide polymorphismtarget capturewheat

Identifiers

PMID28913866
PMCPMC5866950

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.