Evidence map›Paper›PMID 28913707›Full record

ArticlePlant cell reports2017

Endocytic pathways involved in PLGA nanoparticle uptake by grapevine cells and role of cell wall and membrane in size selection.

Cleofe Palocci, Alessio Valletta, Laura Chronopoulou, Livia Donati, Marco Bramosanti, Elisa Brasili, Barbara Baldan, Gabriella Pasqua

Abstract read
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In one paragraph

Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 123 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Cleofe PalocciDepartment of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185, Rome, Italy.
Alessio VallettaDepartment of Environmental Biology, University of Rome La Sapienza, Piazzale A. Moro 5, 00185, Rome, Italy. alessio.valletta@uniroma1.it.ORCID http://orcid.org/0000-0002-8988-1400
Laura ChronopoulouDepartment of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185, Rome, Italy.
Livia DonatiDepartment of Environmental Biology, University of Rome La Sapienza, Piazzale A. Moro 5, 00185, Rome, Italy.
Marco BramosantiDepartment of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185, Rome, Italy.
Elisa BrasiliDepartment of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185, Rome, Italy.
Barbara BaldanDepartment of Biology, University of Padua, Via Ugo Bassi 58/B, 35131, Padua, Italy.
Gabriella PasquaDepartment of Environmental Biology, University of Rome La Sapienza, Piazzale A. Moro 5, 00185, Rome, Italy.
Sapienza University of Rome · ITUniversity of Padua · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messagePLGA NPs' cell uptake involves different endocytic pathways. Clathrin-independent endocytosis is the main internalization route. The cell wall plays a more prominent role than the plasma membrane in NPs' size selection. In the last years, many studies on absorption and cell uptake of nanoparticles by plants have been conducted, but the understanding of the internalization mechanisms is still largely unknown. In this study, polydispersed and monodispersed poly(lactic-co-glycolic) acid nanoparticles (PLGA NPs) were synthesized, and a strategy combining the use of transmission electron microscopy (TEM), confocal analysis, fluorescently labeled PLGA NPs, a probe for endocytic vesicles (FM4-64), and endocytosis inhibitors (i.e., wortmannin, ikarugamycin, and salicylic acid) was employed to shed light on PLGA NP cell uptake in grapevine cultured cells and to assess the role of the cell wall and plasma membrane in size selection of PLGA NPs. The ability of PLGA NPs to cross the cell wall and membrane was confirmed by TEM and fluorescence microscopy. A strong adhesion of PLGA NPs to the outer side of the cell wall was observed, presumably due to electrostatic interactions. Confocal microscopy and treatment with endocytosis inhibitors suggested the involvement of both clathrin-dependent and clathrin-independent endocytosis in cell uptake of PLGA NPs and the latter appeared to be the main internalization pathway. Experiments on grapevine protoplasts revealed that the cell wall plays a more prominent role than the plasma membrane in size selection of PLGA NPs. While the cell wall prevents the uptake of PLGA NPs with diameters over 50 nm, the plasma membrane can be crossed by PLGA NPs with a diameter of 500-600 nm.

Indexed as

Cell WallEndocytosisMicrofluidicsMicroscopy, Electron, TransmissionNanoparticlesVitisCell culturesEndocytosisMicrofluidicsPolymeric nanoparticlesVitis vinifera

Identifiers

PMID28913707
OpenAlexW2755731316

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.