ArticleScientific reports2017
Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 181 citations in OpenAlex.
- Advances in Genome Editing for Plant Disease Resistance Breeding.Plants (Basel, Switzerland) · 2026Review
- MicroRNA networks in rice seeds: unveiling key regulators of development, germination, and rice resilience under climate changes.Journal of experimental botany · 2026Review
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Efficient genome editing by CRISPR-Cas12a in common wheat via shoot apical meristem delivery.Plant physiology · 2025Article
- Multiplex CRISPR-Cas9 editing of chlorophyll biosynthesis genes in chickpea via protoplast and Agrobacterium-mediated transformation.Functional & integrative genomics · 2025Article
- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
- Development of efficient targeted insertion mediated by CRISPR-Cas12a and homology-directed repair in maize.Frontiers in genome editing · 2025Article
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- CRISPR-Cpf1 system and its applications in animal genome editing.Molecular genetics and genomics : MGG · 2024Review
- Cas12a and MAD7, genome editing tools for breeding.Breeding science · 2024Article
- Simple promotion of Cas9 and Cas12a expression improves gene targeting via an all-in-one strategy.Frontiers in plant science · 2024Article
- Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation.Nature communications · 2023Article
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- Current and Prospective Applications of CRISPR-Cas12a in Pluricellular Organisms.Molecular biotechnology · 2023Review
- Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (International journal of molecular sciences · 2022Article
- CRISPR/Cas9-Mediated Targeted DNA Integration: Rearrangements at the Junction of Plant and Plasmid DNA.International journal of molecular sciences · 2022Article
- Modification of ginsenoside saponin composition via the CRISPR/Cas9-mediated knockout of protopanaxadiol 6-hydroxylase gene inJournal of ginseng research · 2022Article
- Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World.Plants (Basel, Switzerland) · 2022Review
- An update on precision genome editing by homology-directed repair in plants.Plant physiology · 2022Article
- State-of-the-Art in CRISPR Technology and Engineering Drought, Salinity, and Thermo-tolerant crop plants.Plant cell reports · 2022Review
36 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise genome editing of plants has the potential to reshape global agriculture through the targeted engineering of endogenous pathways or the introduction of new traits. To develop a CRISPR nuclease-based platform that would enable higher efficiencies of precise gene insertion or replacement, we screened the Cpf1 nucleases from Francisella novicida and Lachnospiraceae bacterium ND2006 for their capability to induce targeted gene insertion via homology directed repair. Both nucleases, in the presence of a guide RNA and repairing DNA template flanked by homology DNA fragments to the target site, were demonstrated to generate precise gene insertions as well as indel mutations at the target site in the rice genome. The frequency of targeted insertion for these Cpf1 nucleases, up to 8%, is higher than most other genome editing nucleases, indicative of its effective enzymatic chemistry. Further refinements and broad adoption of the Cpf1 genome editing technology have the potential to make a dramatic impact on plant biotechnology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.