ArticleScientific reports2017
Meiotic arrest with roscovitine and follicular fluid improves cytoplasmic maturation of porcine oocytes by promoting chromatin de-condensation and gene transcription.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 31 citations in OpenAlex.
- Advances in pig embryo production for cryopreservation, transfer and genome editing.The Journal of reproduction and development · 2026Review
- The combination of rolipram and cilostamide improved the developmental competence of cloned porcine embryos.Scientific reports · 2023Article
- Article
- Article
- Signaling mechanisms and their regulation during in vivo or in vitro maturation of mammalian oocytes.Reproductive biology and endocrinology : RB&E · 2022Review
- Proteomic Exploration of Porcine Oocytes During Meiotic MaturationFrontiers in veterinary science · 2021Article
- Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation.Animals : an open access journal from MDPI · 2020Article
- Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes.Scientific reports · 2020Article
- Effect of roscovitine on developmental competence of small follicle-derived buffalo oocytes.The Indian journal of medical research · 2018Article
- Mechanisms by which in vitro meiotic arrest and sexual maturity improve developmental potential of mouse oocytes.Scientific reports · 2017Article
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Authors and funding
8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The developmental capacity of in vitro matured oocytes is inferior to that of the in vivo matured ones due to insufficient cytoplasmic maturation. Although great efforts were made to accomplish better cytoplasmic maturation by meiotic arrest maintenance (MAM) before in vitro maturation (IVM), limited progress has been achieved in various species. This study showed that MAM of porcine oocytes was better achieved with roscovitine than with dibutyryl cyclic adenosine monophosphate (db-cAMP) or 3-isobutyl-1-methylxanthine. Oocyte developmental competence after IVM was significantly improved following MAM in 199 + FF medium (TCM-199 containing 10% porcine follicular fluid and 25 µM roscovitine) to a level even higher than that in control oocytes matured without pre-MAM. Observations on other markers further confirmed the positive effects of MAM in 199 + FF on oocyte cytoplasmic maturation. During MAM culture in 199 + FF, re-decondensation (RDC) of condensed chromatin occurred, and transcription of genes beneficial to cytoplasmic maturation was evident in some of the oocytes with surrounded nucleoli (SN). However, MAM with db-cAMP neither induced RDC nor improved oocyte developmental potential. Together, the results suggest that MAM in the presence of FF and roscovitine improved the developmental competence of porcine oocytes by promoting a pre-GVBD chromatin de-condensation and expression of beneficial genes.
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