Evidence map›Paper›PMID 28911908›Full record

ArticleExperimental hematology2018

Enhancement of mouse hematopoietic stem/progenitor cell function via transient gene delivery using integration-deficient lentiviral vectors.

Maria E Alonso-Ferrero, Niek P van Til, Kerol Bartolovic, Márcia F Mata, Gerard Wagemaker, Dale Moulding, David A Williams, Christine Kinnon, Simon N Waddington, Michael D Milsom and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental hematology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 7 countries.

Maria E Alonso-FerreroMolecular and Cellular Immunology, University College London Great Ormond Street Institute of Child Health, London, UK.
Niek P van TilDepartment of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
Kerol BartolovicWeatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Márcia F MataDepartment of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
Gerard WagemakerDepartment of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands; Hacettepe University, Stem Cell Research and Development Center, Ankara, Turkey; Raisa Gorbacheva Memorial Research Institute for Pediatric Oncology and Hematology, Saint Petersburg, Russia.
Dale MouldingMolecular and Cellular Immunology, University College London Great Ormond Street Institute of Child Health, London, UK.
David A WilliamsBoston Children's Hospital, Harvard Medical School, Boston, MA, USA.
Christine KinnonMolecular and Cellular Immunology, University College London Great Ormond Street Institute of Child Health, London, UK.
Simon N WaddingtonGene Transfer Technology Group, University College London Institute for Women's Health, London, UK; Antiviral Gene Therapy Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Michael D MilsomExperimental Hematology Group, Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM) and the German Cancer Research Center, Im Neuenheimer Feld 280, Heidelberg, Germany. Electronic address: michael.milsom@Dkfz-Heidelberg.de.
Steven J HoweMolecular and Cellular Immunology, University College London Great Ormond Street Institute of Child Health, London, UK; Gene Transfer Technology Group, University College London Institute for Women's Health, London, UK.
University College London · GBErasmus MC · NLBoston Children's Hospital · USHeidelberg University · DEUniversity of Lisbon · PTUniversity of Oxford · GBUniversity of the Witwatersrand · ZA

Funding

Rac proteins in hematopoietic cell survival and functionR01DK062757 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI WILLIAMS, DAVID A · 2002 to 2016
$5.7M
Biotechnology and Biological Sciences Research Council BB/I00212X/1Medical Research Council G1000709Medical Research Council MR/N026101/1Medical Research Council MR/P026494/1Medical Research Council MR/R015325/1NIDDK NIH HHS R01 DK062757
6 · The paper itself

Abstract

Integration-deficient lentiviruses (IdLVs) deliver genes effectively to tissues but are lost rapidly from dividing cells. This property can be harnessed to express transgenes transiently to manipulate cell biology. Here, we demonstrate the utility of short-term gene expression to improve functional potency of hematopoietic stem and progenitor cells (HSPCs) during transplantation by delivering HOXB4 and Angptl3 using IdLVs to enhance the engraftment of HSPCs. Constitutive overexpression of either of these genes is likely to be undesirable, but the transient nature of IdLVs reduces this risk and those associated with unsolicited gene expression in daughter cells. Transient expression led to increased multilineage hematopoietic engraftment in in vivo competitive repopulation assays without the side effects reported in constitutive overexpression models. Adult stem cell fate has not been programmed previously using IdLVs, but we demonstrate that these transient gene expression tools can produce clinically relevant alterations or be applied to investigate basic biology.

Indexed as

Transduction, GeneticAngiopoietin-Like Protein 3Angiopoietin-like ProteinsAnimalsCell LineageGene Expression RegulationGenes, ReporterGenetic VectorsGraft SurvivalHematopoiesisHematopoietic Stem CellsHematopoietic Stem Cell TransplantationHomeodomain ProteinsHumansK562 CellsLentivirusAngiopoietin-Like Protein 3Angiopoietin-like ProteinsAngptl3 protein, mouseHomeodomain ProteinsHOXB4 protein, humanRecombinant Fusion ProteinsTranscription Factors

Identifiers

PMID28911908
PMCPMC5731634
OpenAlexW2756298327

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.