Evidence map›Paper›PMID 28911769›Full record

ArticleMethods in enzymology2017

Measurement of Kinetic Isotope Effects in an Enzyme-Catalyzed Reaction by Continuous-Flow Mass Spectrometry.

Kenneth M Roberts, Paul F Fitzpatrick

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Kenneth M RobertsUniversity of South Carolina Aiken, Aiken, SC, United States.
Paul F FitzpatrickUniversity of Texas Health Science Center, San Antonio, TX, United States. Electronic address: fitzpatrickp@uthscsa.edu.
The University of Texas Health Science Center at San Antonio · USUniversity of South Carolina Aiken · US

Funding

Mechanisms of FlavoproteinsR01GM058698 · NIGMS · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI FITZPATRICK, PAUL F. · 1999 to 2017
$4.6M
NIGMS NIH HHS R01 GM058698
6 · The paper itself

Abstract

Kinetic isotope effects (KIEs) provide powerful probes of the mechanisms of enzyme-catalyzed reactions. In this chapter, we describe the use of continuous-flow mass spectrometry to determine the deuterium KIE for the enzyme N-acetylpolyamine oxidase based on the ratio of labeled and unlabeled products in mass spectra of whole reaction mixtures.

Indexed as

BiocatalysisDeuteriumEnzyme AssaysKineticsMass SpectrometryOxidoreductases Acting on CH-NH Group DonorsPolyamine OxidaseDeuteriumOxidoreductases Acting on CH-NH Group DonorsPolyamine OxidaseAmine oxidaseContinuous flowEnzymeFlavoproteinIsotope effectMass spectrometryPolyamine oxidase

Identifiers

PMID28911769
PMCPMC6767924
OpenAlexW2744548844

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.