ArticleMethods (San Diego, Calif.)2017
Time-lapsed, large-volume, high-resolution intravital imaging for tissue-wide analysis of single cell dynamics.
Article in Methods (San Diego, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 48 citations in OpenAlex.
- Synchrotron-based multi-scale biological imaging inside biocontainment environments.Journal of synchrotron radiation · 2026Article
- Targeting CSF-1 signaling between tumor cells and macrophages at TMEM doorways inhibits breast cancer dissemination.Oncogene · 2025Article
- Imaging Approaches in Cancer Biology.Cold Spring Harbor perspectives in medicine · 2024Review
- Signaling events at TMEM doorways provide potential targets for inhibiting breast cancer dissemination.bioRxiv : the preprint server for biology · 2024Article
- Recent Trends in Nanocarrier-Based Drug Delivery System for Prostate Cancer.AAPS PharmSciTech · 2024Review
- Stabilized Window for Intravital Imaging of the Murine Pancreas.Journal of visualized experiments : JoVE · 2023Article
- Intravital imaging to study cancer progression and metastasis.Nature reviews. Cancer · 2023Review
- Assessment of MRI to estimate metastatic dissemination risk and prometastatic effects of chemotherapy.NPJ breast cancer · 2022Article
- NR2F1 Is a Barrier to Dissemination of Early-Stage Breast Cancer Cells.Cancer research · 2022Article
- SWIP-a stabilized window for intravital imaging of the murine pancreas.Open biology · 2022Article
- Article
- Immune function and dysfunction are determined by lymphoid tissue efficacy.Disease models & mechanisms · 2022Review
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination.Nature communications · 2021Article
- A Permanent Window for Investigating Cancer Metastasis to the Lung.Journal of visualized experiments : JoVE · 2021Article
- Article
- The Indiana O'Brien Center for Advanced Renal Microscopic Analysis.American journal of physiology. Renal physiology · 2021Review
- Intravital microscopy of dynamic single-cell behavior in mouse mammary tissue.Nature protocols · 2021Article
- The Cellular Organization of the Mammary Gland: Insights From Microscopy.Journal of mammary gland biology and neoplasia · 2021Review
- Intravital Metabolic Autofluorescence Imaging Captures Macrophage Heterogeneity Across Normal and Cancerous Tissue.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Pathologists rely on microscopy to diagnose disease states in tissues and organs. They utilize both high-resolution, high-magnification images to interpret the staining and morphology of individual cells, as well as low-magnification overviews to give context and location to these cells. Intravital imaging is a powerful technique for studying cells and tissues in their native, live environment and can yield sub-cellular resolution images similar to those used by pathologists. However, technical limitations prevent the straightforward acquisition of low-magnification images during intravital imaging, and they are hence not typically captured. The serial acquisition, mosaicking, and stitching together of many high-resolution, high-magnification fields of view is a technique that overcomes these limitations in fixed and ex vivo tissues. The technique however, has not to date been widely applied to intravital imaging as movements caused by the living animal induce image distortions that are difficult to compensate for computationally. To address this, we have developed techniques for the stabilization of numerous tissues, including extremely compliant tissues, that have traditionally been extremely difficult to image. We present a novel combination of these stabilization techniques with mosaicked and stitched intravital imaging, resulting in a process we call Large-Volume High-Resolution Intravital Imaging (LVHR-IVI). The techniques we present are validated and make large volume intravital imaging accessible to any lab with a multiphoton microscope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.