ArticleNucleic acids research2017
A novel nucleoid-associated protein coordinates chromosome replication and chromosome partition.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- GapR stiffens AT-rich and overtwisted DNA leading to differential effects on transcription based on gene context.Nucleic acids research · 2026Article
- Temporospatial control of topoisomerases by essential cellular processes.Current opinion in microbiology · 2024Review
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Three factors ParA, TipN, and DnaA-mediated chromosome replication initiation are contributors of centromere segregation inMolecular biology of the cell · 2024Article
- TipN's involvement with centromere segregation in Caulobacter crescentus.bioRxiv : the preprint server for biology · 2023Article
- Article
- The DNA Recognition Motif of GapR Has an Intrinsic DNA Binding Preference towards AT-rich DNA.Molecules (Basel, Switzerland) · 2021Article
- Article
- DNA-Binding Properties of YbaB, a Putative Nucleoid-Associated Protein FromFrontiers in microbiology · 2021Article
- GapR binds DNA through dynamic opening of its tetrameric interface.Nucleic acids research · 2020Article
- Chromatin Architectural Factors as Safeguards against Excessive Supercoiling during DNA Replication.International journal of molecular sciences · 2020Review
- The Nucleoid-Associated Protein GapR Uses Conserved Structural Elements To Oligomerize and Bind DNA.mBio · 2020Article
- Bacterial chromosome segregation by the ParABS system.Open biology · 2020Review
- Chromosome organization in bacteria: mechanistic insights into genome structure and function.Nature reviews. Genetics · 2020Review
- Structures of GapR reveal a central channel which could accommodate B-DNA.Scientific reports · 2019Article
- Random Chromosome Partitioning in the Polyploid BacteriumG3 (Bethesda, Md.) · 2019Article
- Multilayered control of chromosome replication inBiochemical Society transactions · 2019Review
- Crosstalk Regulation Between Bacterial Chromosome Replication and Chromosome Partitioning.Frontiers in microbiology · 2019Review
- Editorial: DNA Replication Origins in Microbial Genomes, Volume 2.Frontiers in microbiology · 2019Article
- Endogenous and Foreign Nucleoid-Associated Proteins of Bacteria: Occurrence, Interactions and Effects on Mobile Genetic Elements and Host's Biology.Computational and structural biotechnology journal · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We searched for regulators of chromosome replication in the cell cycle model Caulobacter crescentus and found a novel DNA-binding protein (GapR) that selectively aids the initiation of chromosome replication and the initial steps of chromosome partitioning. The protein binds the chromosome origin of replication (Cori) and has higher-affinity binding to mutated Cori-DNA that increases Cori-plasmid replication in vivo. gapR gene expression is essential for normal rapid growth and sufficient GapR levels are required for the correct timing of chromosome replication. Whole genome ChIP-seq identified dynamic DNA-binding distributions for GapR, with the strongest associations at the partitioning (parABS) locus near Cori. Using molecular-genetic and fluorescence microscopy experiments, we showed that GapR also promotes the first steps of chromosome partitioning, the initial separation of the duplicated parS loci following replication from Cori. This separation occurs before the parABS-dependent partitioning phase. Therefore, this early separation, whose mechanisms is not known, coincides with the poorly defined mechanism(s) that establishes chromosome asymmetry: C. crescentus chromosomes are partitioned to distinct cell-poles which develop into replicating and non-replicating cell-types. We propose that GapR coordinates chromosome replication with asymmetry-establishing chromosome separation, noting that both roles are consistent with the phylogenetic restriction of GapR to asymmetrically dividing bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.