ArticleJournal of experimental botany2017
Pyrenoid loss in Chlamydomonas reinhardtii causes limitations in CO2 supply, but not thylakoid operating efficiency.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Super-resolution expansion microscopy reveals nanoscale protein domains and CObioRxiv : the preprint server for biology · 2026Article
- Proteomic analysis of the pyrenoid-traversing membranes of Chlamydomonas reinhardtii reveals novel components.The New phytologist · 2026Article
- Article
- Proteomic analysis of the pyrenoid-traversing membranes ofbioRxiv : the preprint server for biology · 2025Article
- Bestrophin-like protein 4 is involved in photosynthetic acclimation to light fluctuations in Chlamydomonas.Plant physiology · 2024Article
- SAGA1 and MITH1 produce matrix-traversing membranes in the CONature plants · 2024Article
- Chloroplast Methyltransferase Homolog RMT2 is Involved in Photosystem I Biogenesis.bioRxiv : the preprint server for biology · 2024Article
- CAH3 fromCells · 2024Review
- Tools for Measuring Photosynthesis at Different Scales.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The pyrenoid: the eukaryotic CO2-concentrating organelle.The Plant cell · 2023Article
- Converting antimicrobial into targeting peptides reveals key features governing protein import into mitochondria and chloroplasts.Plant communications · 2023Article
- The Chlamydomonas bZIP transcription factor BLZ8 confers oxidative stress tolerance by inducing the carbon-concentrating mechanism.The Plant cell · 2022Article
- Transit Peptides Often Require Downstream Unstructured Sequence for Efficient Chloroplast Import inFrontiers in plant science · 2022Article
- Orchestral manoeuvres in the light: crosstalk needed for regulation of the Chlamydomonas carbon concentration mechanism.Journal of experimental botany · 2021Review
- Rubisco proton production can drive the elevation of COProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The structural basis of Rubisco phase separation in the pyrenoid.Nature plants · 2020Article
- Assembly of the algal COScience advances · 2020Article
- Prospects for Engineering Biophysical COAnnual review of plant biology · 2020Review
- Elucidation and genetic intervention of COJournal of biosciences · 2020Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The pyrenoid of the unicellular green alga Chlamydomonas reinhardtii is a microcompartment situated in the centre of the cup-shaped chloroplast, containing up to 90% of cellular Rubisco. Traversed by a network of dense, knotted thylakoid tubules, the pyrenoid has been proposed to influence thylakoid biogenesis and ultrastructure. Mutants that are unable to assemble a pyrenoid matrix, due to expressing a vascular plant version of the Rubisco small subunit, exhibit severe growth and photosynthetic defects and have an ineffective carbon-concentrating mechanism (CCM). The present study set out to determine the cause of photosynthetic limitation in these pyrenoid-less lines. We tested whether electron transport and light use were compromised as a direct structural consequence of pyrenoid loss or as a metabolic effect downstream of lower CCM activity and resulting CO2 limitation. Thylakoid organization was unchanged in the mutants, including the retention of intrapyrenoid-type thylakoid tubules, and photosynthetic limitations associated with the absence of the pyrenoid were rescued by exposing cells to elevated CO2 levels. These results demonstrate that Rubisco aggregation in the pyrenoid functions as an essential element for CO2 delivery as part of the CCM, and does not play other roles in maintenance of photosynthetic membrane energetics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.