ArticlePLoS pathogens2017
Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 54 citations in OpenAlex.
- Reorganizing the RNA polymerase II complex for replication of an infectious noncoding RNA in vivo.PLoS pathogens · 2026Article
- Diversity and evolution of viroids and viroid-like agents with circular RNA genomes revealed by metatranscriptome mining.Nucleic acids research · 2025Article
- Prevalence, genetic diversity, and molecular detection of the apple hammerhead viroid in Germany.Frontiers in microbiology · 2025Article
- Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication.Plants (Basel, Switzerland) · 2024Review
- Characterisation of the genetic diversity of citrus viroid VII using amplicon sequencing.Archives of virology · 2024Article
- Uncovered diversity of infectious circular RNAs: A new paradigm for the minimal parasites?Npj viruses · 2024Review
- RNA three-dimensional structure drives the sequence organization of potato spindle tuber viroid quasispecies.PLoS pathogens · 2024Article
- Cell-cell communication and initial population composition shape the structure of potato spindle tuber viroid quasispecies.The Plant cell · 2024Article
- Viroids, Satellite RNAs and Prions: Folding of Nucleic Acids and Misfolding of Proteins.Viruses · 2024Article
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- Tobacco mosaic virus movement protein complements a Potato spindle tuber viroid RNA mutant impaired for mesophyll entry but not mutants unable to enter the phloem.PLoS pathogens · 2022Article
- A remodeled RNA polymerase II complex catalyzing viroid RNA-templated transcription.PLoS pathogens · 2022Article
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- A scenario for the emergence of protoviroids in the RNA world and for their further evolution into viroids and viroid-like RNAs by modular recombinations and mutations.Virus evolution · 2022Review
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- Current view and perspectives in viroid replication.Current opinion in virology · 2021Review
- Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid-host interactions.Molecular plant pathology · 2021Article
- Biased Pol II fidelity contributes to conservation of functional domains in the Potato spindle tuber viroid genome.PLoS pathogens · 2020Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutation rates vary by orders of magnitude across biological systems, being higher for simpler genomes. The simplest known genomes correspond to viroids, subviral plant replicons constituted by circular non-coding RNAs of few hundred bases. Previous work has revealed an extremely high mutation rate for chrysanthemum chlorotic mottle viroid, a chloroplast-replicating viroid. However, whether this is a general feature of viroids remains unclear. Here, we have used high-fidelity ultra-deep sequencing to determine the mutation rate in a common host (eggplant) of two viroids, each representative of one family: the chloroplastic eggplant latent viroid (ELVd, Avsunviroidae) and the nuclear potato spindle tuber viroid (PSTVd, Pospiviroidae). This revealed higher mutation frequencies in ELVd than in PSTVd, as well as marked differences in the types of mutations produced. Rates of spontaneous mutation, quantified in vivo using the lethal mutation method, ranged from 1/1000 to 1/800 for ELVd and from 1/7000 to 1/3800 for PSTVd depending on sequencing run. These results suggest that extremely high mutability is a common feature of chloroplastic viroids, whereas the mutation rates of PSTVd and potentially other nuclear viroids appear significantly lower and closer to those of some RNA viruses.
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