ArticlePloS one2017
Potential DNA barcodes for Melilotus species based on five single loci and their combinations.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Sweet Clover (Molecules (Basel, Switzerland) · 2025Article
- A comparative plastome approach enhances the assessment of genetic variation in the Melilotus genus.BMC genomics · 2024Article
- Halophyte Plants as Potential Sources of Anticancer Agents: A Comprehensive Review.Pharmaceutics · 2022Review
- Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis.Plant biotechnology journal · 2022Article
- Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics.BMC plant biology · 2022Article
- NAC and MYB Families and Lignin Biosynthesis-Related Members Identification and Expression Analysis inPlants (Basel, Switzerland) · 2021Article
- LAITOR4HPC: A text mining pipeline based on HPC for building interaction networks.BMC bioinformatics · 2020Article
- Expression of Concern: Potential DNA barcodes for Melilotus species based on five single loci and their combinations.PloS one · 2020Article
- Retraction: Genetic variation and diversity in 199 Melilotus accessions based on a combination of 5 DNA sequences.PloS one · 2020Article
- Coumarin Content, Morphological Variation, and Molecular Phylogenetics of Melilotus.Molecules (Basel, Switzerland) · 2018Article
- Article
Corrections and comments
- Expression of concern · 2020-04-20Breach of Policy by Author · Concerns/Issues about Data ·
- Expression of concern
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As an important green manure crop, diverse Melilotus species have different values as feed and medicine. To identify different Melilotus species, we examined the efficiency of five candidate regions as barcodes, including the internal transcribed spacer (ITS) and two chloroplast loci, rbcL and matK, and two non-coding loci, trnH-psbA and trnL-F. In total, 198 individuals from 98 accessions representing 18 Melilotus species were sequenced for these five potential barcodes. Based on inter-specific divergence, we analysed sequences and confirmed that each candidate barcode was able to identify some of the 18 species. The resolution of a single barcode and its combinations ranged from 33.33% to 88.89%. Analysis of pairwise distances showed that matK+rbcL+trnL-F+trnH-psbA+ITS (MRTPI) had the greatest value and rbcL the least. Barcode gap values and similarity value analyses confirmed these trends. The results indicated that an ITS region, successfully identifying 13 of 18 species, was the most appropriate single barcode and that the combination of all five potential barcodes identified 16 of the 18 species. We conclude that MRTPI is the most effective tool for Melilotus species identification. Taking full advantage of the barcode system, a clear taxonomic relationship can be applied to identify Melilotus species and enhance their practical production.
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