ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2017
Calculation of Glucose Dose for Intraperitoneal Glucose Tolerance Tests in Lean and Obese Mice.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
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- The intestinal permeability marker FITC-dextran 4kDa should be dosed according to lean body mass in obese mice.Nutrition & diabetes · 2023Article
- Improvement in insulin sensitivity and prevention of high fat diet-induced liver pathology using a CXCR2 antagonist.Cardiovascular diabetology · 2022Article
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- Comparison of area under the curve in various models of diabetic rats receiving chronic medication.Archives of medical science : AMS · 2022Article
- Resveratrol affects the expression of uric acid transporter by improving inflammation.Molecular medicine reports · 2021Article
- Autoimmune activation of the GnRH receptor induces insulin resistance independent of obesity in a female rat model.Physiological reports · 2021Article
- BAM15-mediated mitochondrial uncoupling protects against obesity and improves glycemic control.EMBO molecular medicine · 2020Article
- A comprehensive and comparative phenotypic analysis of the collaborative founder strains identifies new and known phenotypes.Mammalian genome : official journal of the International Mammalian Genome Society · 2020Article
- Guidelines and Considerations for Metabolic Tolerance Tests in Mice.Diabetes, metabolic syndrome and obesity : targets and therapy · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucose tolerance tests are used frequently in nonclinical research with laboratory animals, for example during characterization of obese phenotypes. Despite published standard operating procedures for glucose tolerance tests in rodents, how glucose doses should be calculated when obese and lean animals are compared is not well documented. Typically the glucose dose is calculated as 2 g/kg body weight, regardless of body composition. With this approach, obese mice receive larger glucose doses than do lean animals, potentially leading to overestimation of glucose intolerance in obese animals. In this study, we performed intraperitoneal glucose tolerance tests in mice with diet-induced obesity and their lean controls, with glucose doses based on either the total body weight or the lean body mass of the animals. To determine glucose tolerance, we determined the blood glucose AUC during the glucose tolerance test. We found that the blood glucose AUC was increased significantly in obese mice compared with lean mice by 75% on average when glucose was dosed according to the lean body mass and by 87% when the glucose dose was calculated according to total body weight. Therefore, mice with diet-induced obesity were approximately equally glucose intolerant between the 2 dose-calculation protocols. However, we recommend calculating the glucose dose according to the lean body mass of the mice, because doing so eliminates the concern regarding overdosing of obese animals.
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Identifiers
28905722PMC5250502W2981237765What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.