Evidence map›Paper›PMID 28905518›Full record

ArticleMicrobial biotechnology2017

Production of a novel medium chain length poly(3-hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold.

Pooja Basnett, Barbara Lukasiewicz, Elena Marcello, Harpreet K Gura, Jonathan C Knowles, Ipsita Roy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Pooja BasnettFaculty of Science and Technology, University of Westminster, London, UK.
Barbara LukasiewiczFaculty of Science and Technology, University of Westminster, London, UK.
Elena MarcelloFaculty of Science and Technology, University of Westminster, London, UK.
Harpreet K GuraAarhus University, Denmark.
Jonathan C KnowlesEastman Dental Institute, University College London, London, UK.
Ipsita RoyFaculty of Science and Technology, University of Westminster, London, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocinaCH50, for the production of PHAs. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate) or P(3HHx-3HO-3HD-3HDD). P(3HHx-3HO-3HD-3HDD) was further characterized and evaluated for its use as a tissue engineering scaffold (TES). This study demonstrated that P(3HHx-3HO-3HD-3HDD) was biocompatible with the C2C12 (myoblast) cell line. In fact, the % cell proliferation of C2C12 on the P(3HHx-3HO-3HD-3HDD) scaffold was 72% higher than the standard tissue culture plastic confirming that this novel PHA was indeed a promising new material for soft tissue engineering.

Indexed as

AnimalsCell LineCell ProliferationMiceMolecular StructureMyoblastsPolyhydroxyalkanoatesPseudomonas mendocinaTissue EngineeringTissue ScaffoldsWaste ProductsPolyhydroxyalkanoatesWaste Products

Identifiers

PMID28905518
PMCPMC5658593

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.