ArticlePlant cell reports2017
Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae.
Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- RUBY-BD: a highly efficient visual reporter system for assessing bidirectional promoter activity.Planta · 2026Article
- A century of advances in molecular genetics and breeding for sustainable resistance to rice blast disease.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Gene pyramiding ofMolecular breeding : new strategies in plant improvement · 2024Article
- Genetic and molecular analysis of leaf blast resistance in Tetep derived line RIL4 and its relationship to genes at Pita/PitaScientific reports · 2023Article
- The northern corn leaf blight resistance gene Ht1 encodes an nucleotide-binding, leucine-rich repeat immune receptor.Molecular plant pathology · 2023Article
- iTRAQ based proteomic analysis of rice lines having single or stacked blast resistance genes:Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Plant and pathogen genomics: essential approaches for stem rust resistance gene stacks in wheat.Frontiers in plant science · 2023Review
- Understanding the Dynamics of Blast Resistance in Rice-Journal of fungi (Basel, Switzerland) · 2022Review
- Pyramiding the antimicrobial PR1aCB and AATCB genes in 'Tarocco' blood orange (Citrus sinensis Osbeck) to enhance citrus canker resistance.Transgenic research · 2021Article
- Characterization and Evaluation of Transgenic Rice Pyramided with the Pi Genes Pib, Pi25 and Pi54.Rice (New York, N.Y.) · 2021Article
- Eighty years of gene-for-gene relationship and its applications in identification and utilization ofJournal of genetics · 2021Review
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Blast resistance gene Pi54 over-expressed in rice to understand its cellular and sub-cellular localization and response to different pathogens.Scientific reports · 2020Article
- Breeding Strategies and Challenges in the Improvement of Blast Disease Resistance in Finger Millet. A Current Review.Frontiers in plant science · 2020Review
- Nanobiolistics: An Emerging Genetic Transformation Approach.Methods in molecular biology (Clifton, N.J.) · 2020Article
- First Draft Genome Sequence of a Pearl Millet Blast Pathogen, Magnaporthe grisea Strain PMg_Dl, Obtained Using PacBio Single-Molecule Real-Time and Illumina NextSeq 500 Sequencing.Microbiology resource announcements · 2019Article
- Contribution of recent technological advances to future resistance breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Structural and Functional Analysis of a Bidirectional Promoter fromInternational journal of molecular sciences · 2018Article
- Prospects of Understanding the Molecular Biology of Disease Resistance in Rice.International journal of molecular sciences · 2018Review
- Stacking of blast resistance orthologue genes in susceptible indica rice line improves resistance against3 Biotech · 2018Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThis is the first report of stacking two major blast resistance genes in blast susceptible rice variety using co-transformation method to widen the resistance spectrum against different isolates of Magnaporthe oryzae. Single resistance (R-) gene mediated approach for the management of rice blast disease has met with frequent breakdown in resistance response. Besides providing the durable resistance, gene pyramiding or stacking also imparts broad spectrum resistance against plant pathogens, including rice blast. In the present study, we stacked two R-genes; Pi54 and Pi54rh having broad spectrum resistance against multiple isolates of Magnaporthe oryzae (M. oryzae). Both Pi54 and Pi54rh expressed under independent promoters were transferred into the blast susceptible japonica rice Taipei 309 (TP309) using particle gun bombardment method. Functional complementation analysis of stacked transgenic rice lines showed higher level of resistance to a set of highly virulent M. oryzae isolates collected from different rice growing regions. qRT-PCR analysis has shown M. oryzae induced expression of both the R-genes in stacked transgenic lines. The present study also demonstrated the effectiveness of the strategy for rapid single step gene stacking using co-transformation approach to engineer durable resistance against rice blast disease and also this is the first report in which two blast R-genes are stacked together using co-transformation approach. The two-gene-stacked transgenic line developed in this study can be used further to understand the molecular aspects of defense-related pathways vis-a-vis single R-gene containing transgenic lines.
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