ArticleScientific reports2017
An in planta biolistic method for stable wheat transformation.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 72 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
72 citing papers in PubMed, 133 citations in OpenAlex.
- Genotype-flexible plant genetic transformation: advances and prospects.Frontiers in plant science · 2026Review
- Overcoming Recalcitrance: A Review of Regeneration Methods and Challenges in Roses.Plants (Basel, Switzerland) · 2025Review
- Efficient genome editing by CRISPR-Cas12a in common wheat via shoot apical meristem delivery.Plant physiology · 2025Article
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
- A review of factors affecting the success of geminivirus infectious clones.Plant cell reports · 2025Review
- Enhancing biolistic plant transformation and genome editing with a flow guiding barrel.Nature communications · 2025Article
- Genome editing in maize and sorghum: A comprehensive review of CRISPR/Cas9 and emerging technologies.The plant genome · 2025Review
- Pigments to precision: RUBY aiding genetic transformation and genome editing in wheat and barley.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- In planta genome editing in citrus facilitated by co-expression of CRISPR/Cas and developmental regulators.The Plant journal : for cell and molecular biology · 2025Article
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- BrSQE1 and the ethylene signaling pathway suppress cell division to regulate plant size in Chinese cabbage (Frontiers in plant science · 2025Article
- Review
- A long shelf-life melon created via CRISPR/Cas9 RNP-basedFrontiers in genome editing · 2025Article
- DNA delivery into plant tissues using carbon dots made from citric acid and β-alanine.Frontiers in chemistry · 2025Article
- Development of high-throughput tissue culture-free plant transformation systems.The Plant journal : for cell and molecular biology · 2025Review
- Review
- Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.Plant cell reports · 2024Review
- Nuclear integration of MYB36 and APX-1 genes impart heat tolerance in wheat.Functional & integrative genomics · 2024Article
- Plant-based vaccines against viral hepatitis: A panoptic review.World journal of virology · 2024Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The currently favoured method for wheat (Triticum aestivum L.) transformation is inapplicable to many elite cultivars because it requires callus culture and regeneration. Here, we developed a simple, reproducible, in planta wheat transformation method using biolistic DNA delivery without callus culture or regeneration. Shoot apical meristems (SAMs) grown from dry imbibed seeds were exposed under a microscope and subjected to bombardment with different-sized gold particles coated with the GFP gene construct, introducing DNA into the L2 cell layer. Bombarded embryos were grown to mature, stably transformed T
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.