ArticleScientific reports2017
RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances triacylglycerol biosynthesis in the oleaginous marine alga Nannochloropsis salina.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Pooled it
- The life cycle of fatty acids in oleaginous stramenopiles: from synthesis to maturation and beyond.BMC plant biology · 2026Review
- Enhancing vibriosis vaccination in fish: genetically modified Nannochloropsis sp. as an oral vaccine carrier.World journal of microbiology & biotechnology · 2026Article
- Integrative Multi-Omics Analyses Reveal the Global Regulation Network of the MicroalgaBiology · 2025Article
- Living photosynthetic micro/nano-platforms: Engineering unicellular algae for biomedical applications.Bioactive materials · 2025Review
- Enhancing lipid production inFrontiers in microbiology · 2025Article
- Review
- Review
- Advances in Genetic Engineering in Improving Photosynthesis and Microalgal Productivity.International journal of molecular sciences · 2023Review
- Biochemical and Morphological Changes Triggered by Nitrogen Stress in the Oleaginous MicroalgaMicroorganisms · 2022Article
- Exploration of Microalgal Species for Nutrient Removal from Anaerobically Digested Swine Wastewater and Potential Lipids Production.Microorganisms · 2021Article
- Current perspectives on integrated approaches to enhance lipid accumulation in microalgae.3 Biotech · 2021Review
- Consequences of Mixotrophy on Cell Energetic Metabolism inFrontiers in plant science · 2021Article
- Improving lipid productivity by engineering a control-knob gene in the oleaginous microalgaMetabolic engineering communications · 2020Article
- Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds.Marine drugs · 2020Review
- Integration of proteome and transcriptome refines key molecular processes underlying oil production inBiotechnology for biofuels · 2020Article
- Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High COFrontiers in plant science · 2020Article
- Principles of plastid reductive evolution illuminated by nonphotosynthetic chrysophytes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- High-efficiency nuclear transformation of the microalgaeBiotechnology for biofuels · 2019Article
- Heterologous synthesis of chlorophyllBiotechnology for biofuels · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oleaginous microalgae have been emerging as the third-generation feedstocks for biofuel production. Genetic manipulation for improving triacylglycerol (TAG) accumulation represents a promising approach towards the economics of microalgal biofuels. Acetyl-CoA, the essential carbon precursor for de novo fatty acid biosynthesis, can be derived from pyruvate catalyzed by pyruvate dehydrogenase, which is negatively regulated by pyruvate dehydrogenase kinase (PDK). In the present study, we characterized a PDK gene (NsPDK) from Nannochloropsis salina. Subcellular localization assay assisted by green fluorescence protein (GFP) fusion indicated the localization of NsPDK in mitochondria of N. salina cells. NsPDK knockdown via RNA interference strategy attenuated NsPDK expression at the mRNA level and its enzymatic activity in vivo, leading to faster TAG accumulation without compromising cell growth under high light stress conditions. Interestingly, the TAG increase was accompanied by a decline in membrane polar lipids. NsPDK knockdown also altered fatty acid profile in N. salina. Furthermore, transcriptional analysis suggested that the carbon metabolic pathways might be influenced by NsPDK knockdown leading to diverted carbon flux towards TAG synthesis. Taken together, our results demonstrate the role of NsPDK in regulating TAG accumulation and provide valuable insights into future manipulation of oleaginous microalgae for improving biofuel production.
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