ArticleMolecular biology of the cell2017
Hemodynamic forces can be accurately measured in vivo with optical tweezers.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Review
- Macrophages Mediate Mesoscale Brain Mechanical Homeostasis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Macrophage mediated mesoscale brain mechanical homeostasis mechanically imaged via optical tweezers and Brillouin microscopybioRxiv : the preprint server for biology · 2024Article
- Evidence and therapeutic implications of biomechanically regulated immunosurveillance in cancer and other diseases.Nature nanotechnology · 2024Review
- A cell-and-plasma numerical model reveals hemodynamic stress and flow adaptation in zebrafish microvessels after morphological alteration.PLoS computational biology · 2023Article
- YAP localization mediates mechanical adaptation of human cancer cells during extravasationbioRxiv : the preprint server for biology · 2023Article
- Mechanobiology of cancer cell responsiveness to chemotherapy and immunotherapy: Mechanistic insights and biomaterial platforms.Advanced drug delivery reviews · 2023Review
- On non-Kolmogorov turbulence in blood flow and its possible role in mechanobiological stimulation.Scientific reports · 2022Article
- 3D single cell migration driven by temporal correlation between oscillating force dipoles.eLife · 2022Article
- Application of optical tweezers in cardiovascular research: More than just a measuring tool.Frontiers in bioengineering and biotechnology · 2022Review
- Soft Polymer-Based Technique for Cellular Force Sensing.Polymers · 2021Review
- Impairing flow-mediated endothelial remodeling reduces extravasation of tumor cells.Scientific reports · 2021Article
- Spectrum Degradation of Hippocampal LFP During Euthanasia.Frontiers in systems neuroscience · 2021Article
- Fluids and their mechanics in tumour transit: shaping metastasis.Nature reviews. Cancer · 2020Review
- Quantifying the Local Mechanical Properties of Cells in a Fibrous Three-Dimensional Microenvironment.Biophysical journal · 2019Article
- Perspective: The role of mechanobiology in the etiology of brain metastasis.APL bioengineering · 2018Article
- Manipulation of Biological Cells Using a Robot-Aided Optical Tweezers System.Micromachines · 2018Review
- Biomechanical Characterization at the Cell Scale: Present and Prospects.Frontiers in physiology · 2018Review
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Force sensing and generation at the tissue and cellular scale is central to many biological events. There is a growing interest in modern cell biology for methods enabling force measurements in vivo. Optical trapping allows noninvasive probing of piconewton forces and thus emerged as a promising mean for assessing biomechanics in vivo. Nevertheless, the main obstacles lie in the accurate determination of the trap stiffness in heterogeneous living organisms, at any position where the trap is used. A proper calibration of the trap stiffness is thus required for performing accurate and reliable force measurements in vivo. Here we introduce a method that overcomes these difficulties by accurately measuring hemodynamic profiles in order to calibrate the trap stiffness. Doing so, and using numerical methods to assess the accuracy of the experimental data, we measured flow profiles and drag forces imposed to trapped red blood cells of living zebrafish embryos. Using treatments enabling blood flow tuning, we demonstrated that such a method is powerful in measuring hemodynamic forces in vivo with accuracy and confidence. Altogether this study demonstrates the power of optical tweezing in measuring low range hemodynamic forces in vivo and offers an unprecedented tool in both cell and developmental biology.
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