ArticleCancer research2017
Optical Coherence Tomography Detects Necrotic Regions and Volumetrically Quantifies Multicellular Tumor Spheroids.
Article in Cancer research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 110 citations in OpenAlex.
- Longitudinal drug response assessment of tumor organoids based on optical attenuation coefficient and multi-dimensional morphological characterization.Biomedical optics express · 2026Article
- Evaluation of a human 3D multi-spheroid model derived from SH-SY5Y cells for cytotoxicity testing.Scientific reports · 2026Article
- 3D-Printing-Assisted, Microfabricated Devices Reveal Hierarchical and Temporal Mechanosensing in High-Density Fibroblast Culture.ACS nano · 2026Article
- High-speed full-field swept-source dynamic optical coherence tomography enabled by neural-network-based image generation.Biomedical optics express · 2026Article
- An overview of the developments in 3D cancer cell models, assay techniques, and imaging modalities.Human cell · 2026Review
- Optical coherence photoacoustic microscopy for 3D cancer model imaging with AI-assisted organoid analysis.Light, science & applications · 2026Article
- Longitudinal investigation of prostate tumor spheroid proliferation with dynamic line-field optical coherence tomography.Biomedical optics express · 2026Article
- Advanced imaging strategies in cardiac organoids: bridging the gap between structural complexity and functional analysis.Cardiovascular diabetology · 2026Review
- Evaluating the Efficacy of Mebendazole Repurposing for Ovarian Cancer Therapy Using Optical Coherence Tomography.Journal of biophotonics · 2026Article
- A 3-dimensional Resnet model for assessment of drug efficacy in 3D cancer models using optical coherence tomography.PloS one · 2026Article
- Machine learning-supported identification of necrosis in colorectal and pancreatic cancer spheroids.PloS one · 2026Article
- Ferroptosis Enhances T Lymphocyte Infiltration into Glioblastoma Spheroids.Antioxidants (Basel, Switzerland) · 2025Article
- Navigating Cancer Treatment: A Journey from 2D to 3D Cancer Models and Nanoscale Therapies.ACS pharmacology & translational science · 2025Review
- A modular and reconfigurable microfluidic device for culturing spheroids under continuous perfusion.APL bioengineering · 2025Article
- High-content confocal analysis of tumorigenesis, cancer stem cells, and drug response in 3D cholangiocarcinoma cultures.Scientific reports · 2025Article
- Quantitative evaluation of the site-dependent cell viability in three-dimensional hepatocyte spheroids based on dynamic optical coherence tomography.Journal of biomedical optics · 2025Article
- Numerical modeling of oxygen diffusion in tissue spheroids undergoing fusion using function representation and finite volumes.Scientific reports · 2025Article
- Dual-parameter tomographic imaging of attenuation and backscattering coefficients for quantitative evaluation of immune cell-mediated cytotoxicity in tumor spheroids.Theranostics · 2025Article
- Small adipose-derived mesenchymal stromal cells exhibit longer telomeres and enhanced regenerative potential.Frontiers in bioengineering and biotechnology · 2025Article
- Silk-Ovarioids: establishment and characterization of a human ovarian primary cell 3D-model system.Human reproduction open · 2025Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Three-dimensional (3D) tumor spheroid models have gained increased recognition as important tools in cancer research and anticancer drug development. However, currently available imaging approaches used in high-throughput screening drug discovery platforms, for example, bright-field, phase contrast, and fluorescence microscopies, are unable to resolve 3D structures deep inside (>50 μm) tumor spheroids. In this study, we established a label-free, noninvasive optical coherence tomography (OCT) imaging platform to characterize 3D morphologic and physiologic information of multicellular tumor spheroids (MCTS) growing from approximately 250 to 600 μm in height over 21 days. In particular, tumor spheroids of two cell lines, glioblastoma (U-87MG) and colorectal carcinoma (HCT116), exhibited distinctive evolutions in their geometric shapes at late growth stages. Volumes of MCTS were accurately quantified using a voxel-based approach without presumptions of their geometries. In contrast, conventional diameter-based volume calculations assuming perfect spherical shape resulted in large quantification errors. Furthermore, we successfully detected necrotic regions within these tumor spheroids based on increased intrinsic optical attenuation, suggesting a promising alternative of label-free viability tests in tumor spheroids. Therefore, OCT can serve as a promising imaging modality to characterize morphologic and physiologic features of MCTS, showing great potential for high-throughput drug screening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.