ArticleMicrobial cell factories2017
A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- Unraveling the regulatory dynamics of bidirectional promoters for modulating gene co-expression and metabolic flux in Saccharomyces cerevisiae.Nucleic acids research · 2025Article
- Yeast vaccine production platform for human and animal infectious diseases.Frontiers in immunology · 2025Review
- Chances and drawbacks of derepressed recombinant enzyme production in continuous cultivations withFrontiers in bioengineering and biotechnology · 2025Article
- Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Phaffii.Microbial cell factories · 2024Article
- Review
- Review
- Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9.Microbial cell factories · 2021Article
- Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris.Microbial cell factories · 2021Article
- Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins.Journal of cellular physiology · 2020Review
- Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview.Foods (Basel, Switzerland) · 2020Review
- Characterization of a Bi-directional Promoter OtrRp Involved in Oxytetracycline Biosynthesis.Current microbiology · 2019Article
- Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.Nature communications · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; P
resultsWe characterized a recombinant P. pastoris strain, carrying the novel bi-directional promoter system, during growth and production in three dynamic bioreactor cultivations. We cloned the model enzyme cellobiohydralase downstream of either promoter and applied different feeding strategies to determine the physiological boundaries of the strain. We succeeded in demonstrating tunability of recombinant protein production solely in response to the different feeding strategies and identified a mixed feed regime allowing highest productivity.
conclusionIn this feasibility study, we present the first controlled bioreactor experiments with a recombinant P. pastoris strain carrying a novel bi-directional promotor combination of a catalase promoter variant (P
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