ArticleBMC infectious diseases2017
A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION™ nanopore sequencer.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Machine Learning for CRISPR-Based Diagnostics.International journal of molecular sciences · 2026Review
- Assessment of automated loop-mediated isothermal amplification-(LAMP-)based xenomonitoring for Plasmodium spp. in Anopheles mosquitoes.Malaria journal · 2026Article
- Metagenomic Next-Generation Sequencing in Infectious Diseases: Clinical Applications, Translational Challenges, and Future Directions.Diagnostics (Basel, Switzerland) · 2025Review
- Asymptomatic/submicroscopicParasite epidemiology and control · 2025Article
- The utility of integrating nanopore sequencing into routine HIV-1 drug resistance surveillance.Microbial genomics · 2025Review
- Advances in Malaria Diagnostic Methods in Resource-Limited Settings: A Systematic Review.Tropical medicine and infectious disease · 2024Review
- Multiplexing LAMP Assays: A Methodological Review and Diagnostic Application.International journal of molecular sciences · 2024Review
- Portable nanopore-sequencing technology: Trends in development and applications.Frontiers in microbiology · 2023Review
- Application of a low-cost, specific, and sensitive loop-mediated isothermal amplification (LAMP) assay to detect Plasmodium falciparum imported from Africa.Molecular and biochemical parasitology · 2022Article
- Whole Genome Sequencing Contributions and Challenges in Disease Reduction Focused on Malaria.Biology · 2022Review
- Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections.Frontiers in cellular and infection microbiology · 2022Article
- Nanopore sequencing technology and its application in plant virus diagnostics.Frontiers in microbiology · 2022Review
- Genetic surveillance for monitoring the impact of drug use on Plasmodium falciparum populations.International journal for parasitology. Drugs and drug resistance · 2021Review
- Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP.Malaria journal · 2021Article
- Plasmodium knowlesi detection methods for human infections-Diagnosis and surveillance.Advances in parasitology · 2021Article
- Diagnostic Methods for Non-Falciparum Malaria.Frontiers in cellular and infection microbiology · 2021Review
- Portable sequencer in the fight against infectious disease.Journal of human genetics · 2020Review
- Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria.Sensors (Basel, Switzerland) · 2019Review
- Poor Diagnostic Performance of a Species-Specific Loop-Mediated Isothermal Amplification (LAMP) Platform for Malaria.European journal of microbiology & immunology · 2018Article
- Antimicrobial resistance prediction and phylogenetic analysis of Neisseria gonorrhoeae isolates using the Oxford Nanopore MinION sequencer.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
14 authors at 8 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA simple and accurate molecular diagnostic method for malaria is urgently needed due to the limitations of conventional microscopic examination. In this study, we demonstrate a new diagnostic procedure for human malaria using loop mediated isothermal amplification (LAMP) and the MinION™ nanopore sequencer.
methodsWe generated specific LAMP primers targeting the 18S-rRNA gene of all five human Plasmodium species including two P. ovale subspecies (P. falciparum, P. vivax, P. ovale wallikeri, P. ovale curtisi, P. knowlesi and P. malariae) and examined human blood samples collected from 63 malaria patients in Indonesia. Additionally, we performed amplicon sequencing of our LAMP products using MinION™ nanopore sequencer to identify each Plasmodium species.
resultsOur LAMP method allowed amplification of all targeted 18S-rRNA genes of the reference plasmids with detection limits of 10-100 copies per reaction. Among the 63 clinical samples, 54 and 55 samples were positive by nested PCR and our LAMP method, respectively. Identification of the Plasmodium species by LAMP amplicon sequencing analysis using the MinION™ was consistent with the reference plasmid sequences and the results of nested PCR.
conclusionsOur diagnostic method combined with LAMP and MinION™ could become a simple and accurate tool for the identification of human Plasmodium species, even in resource-limited situations.
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